# Commission Directive 2006/56/EC of 12 June 2006 amending the Annexes to Council Directive 93/85/EEC on the control of potato ring rot, Division 9.1: Nutritional and enzymatic identification tests

Extent: E+W+S+N.I.

Determine the following phenotypic properties which are universally present or absent in C. m. subsp. sepedonicus, according to the methods of Lelliott and Stead (1987), Klement et al. (1990), Schaad (2001), Anonymous (1987).
All media should be incubated at 21 °C and examined after six days. If no growth has occurred, incubate for up to 20 days.
All tests must include a known C. m. subsp.sepedonicus control. Nutritional and physiological tests must be made using inocula from nutrient agar subcultures. Morphological comparisons must be made from nutrient dextrose agar cultures.
Tests
Expected result
Oxidation/Fermentation (O/F) test
Inert or weakly oxidative
Oxidase activity
–
Growth at 37 °C
–
Urease activity
–
Aesculin hydrolysis
+
Starch hydrolysis
– or weak
Tolerance of 7 % NaCl
–
Indole production
–
Catalase activity
+
H2S production
–
Citrate utilization
–
Gelatin liquefaction
–
Acid glycerol
–
Acid from lactose
– or weak
Acid from rhamnose
–
Acid from salicin
–
Gram stain (Appendix 9)
+

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Source: https://www.legislation.gov.uk/eudr/2006/56/annex/I/division/9/division/9.1 (the official version)
Page: https://openlegislation.uk/eu-directive-2006-56/annex-I/division-9/division-9.1
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.