# Commission Directive 2006/56/EC of 12 June 2006 amending the Annexes to Council Directive 93/85/EEC on the control of potato ring rot, Division 9.5: Fatty acid profiling (FAP)

Extent: E+W+S+N.I.

(a) Grow the culture on trypticase soy agar (Oxoid) for 72 hours at 21 °C (+/– 1°).
(b) Apply an appropriate FAP procedure (Janse, 1991; Stead, 1992).
(c) A positive FAP test is achieved if the profile of the presumptive culture is identical to that of the positive control. The presence of characteristic fatty acids are 15:1 Anteiso A, 15:0 Iso, 15:0 Anteiso, 16:0 Iso, 16:0 and 17:0 Anteiso is highly indicative of C. m. sepedonicus. Other genera such as Curtobacterium, Arthrobacter and Micrococcus also have some of these acids but 15:1 Anteiso A is a rare acid in these bacteria but occurs in all Clavibacter spp. at between 1 to 5 %. In C. m. sepedonicus the value is usually around 5 %.

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Source: https://www.legislation.gov.uk/eudr/2006/56/annex/I/division/9/division/9.5 (the official version)
Page: https://openlegislation.uk/eu-directive-2006-56/annex-I/division-9/division-9.5
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.