# Council Directive 2009/156/EC of 30 November 2009 on animal health conditions governing the movement and importation from third countries of equidae (codified version) (Text with EEA relevance), Division 2: Blocking ELISA for the detection of antibodies to African horse sickness virus (AHSV)

Extent: E+W+S+N.I.

The competitive blocking ELISA is designed to detect specific AHSV antibodies in sera from animals of any equine species, i.e. horses, donkeys, zebra and their crosses, preventing the problem of specificity experienced occasionally using the indirect ELISAs.
The principle of the test is the blocking of the reaction between the recombinant VP7 protein absorbed to the ELISA plate and a conjugated AHS-VP7 specific monoclonal antibody (Mab). Antibody in the test sera will block the reaction between the antigen and the Mab resulting in a reduction in colour. Because the Mab is directed against the VP7, the assay will give a high level of sensitivity and specificity.
The competitive blocking ELISA is commercially available.

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Source: https://www.legislation.gov.uk/eudr/2009/156/annex/IV/part/A/division/2 (the official version)
Page: https://openlegislation.uk/eu-directive-2009-156/annex-IV/part-A/division-2
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.