# Council Directive 2009/156/EC of 30 November 2009 on animal health conditions governing the movement and importation from third countries of equidae (codified version) (Text with EEA relevance), Division 2.1.2.1: Serum samples to be tested, and positive and negative control sera, are diluted 1 in 5 in diluent containing 0,35 M NaCl, 0,05 % (v/v) Tween 20 and 0,1 % Kathon, 100 μl per well. Incubate for 1 hour at 37 °C.

Extent: E+W+S+N.I.

For titration, make a twofold dilution series of the test sera from 1 in 10 to 1 in 280 across 8 wells (100 μl/well), one serum per plate column, and do the same with positive and negative controls. Incubate for 1 hour at 37 °C.

---
Source: https://www.legislation.gov.uk/eudr/2009/156/annex/IV/part/A/division/2/division/2.1/division/2.1.2/division/2.1.2.1 (the official version)
Page: https://openlegislation.uk/eu-directive-2009-156/annex-IV/part-A/division-2/division-2.1/division-2.1.2/division-2.1.2.1
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.