{
  "url": "https://openlegislation.uk/eu-directive-2009-156/annex-IV/part-A/division-2/division-2.1/division-2.1.4",
  "json_url": "https://openlegislation.uk/eu-directive-2009-156/annex-IV/part-A/division-2/division-2.1/division-2.1.4.json",
  "legislation": "eudr/2009/156",
  "legislation_title": "Council Directive 2009/156/EC of 30 November 2009 on animal health conditions governing the movement and importation from third countries of equidae (codified version) (Text with EEA relevance)",
  "legislation_url": "https://openlegislation.uk/eu-directive-2009-156",
  "path": "annex/IV/part/A/division/2/division/2.1/division/2.1.4",
  "kind": "division",
  "number": "2.1.4",
  "heading": "Chromogen/Substrate",
  "text": "Add 100 μl/well chromogen/substrate solution, i.e. 1 ml of ABTS (2,2′-Azino-bis-[3-ethylbenzothiazoline-6-sulphonic acid]) 5 mg/ml + 9 ml of substrate buffer (0,1 M Phosphate-Citrate buffer of pH 4 containing 0,03 % H 2 O 2 ), and incubate for 10 minutes at room temperature. Colour development is stopped by adding 100 μl/well of 2 % (w/v) SDS (sodium dodecyl sulphate).",
  "extent": "E+W+S+N.I.",
  "status": null,
  "version": "current",
  "annotations": [
    {
      "type": "F",
      "label": "F1",
      "text": "Substituted by Commission Implementing Decision (EU) 2016/1840 of 14 October 2016 amending Annex IV to Council Directive 2009/156/EC as regards methods for African horse sickness diagnosis (notified under document C(2016) 6509) (Text with EEA relevance)."
    }
  ],
  "outstanding_effects": [],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/eudr/2009/156/annex/IV/part/A/division/2/division/2.1/division/2.1.4",
    "retrieved_at": "2026-11-01T15:22:04.142412+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}