# Council Directive 2009/156/EC of 30 November 2009 on animal health conditions governing the movement and importation from third countries of equidae (codified version) (Text with EEA relevance), Division 2.1.4: Chromogen/Substrate

Extent: E+W+S+N.I.

Add 100 μl/well chromogen/substrate solution, i.e. 1 ml of ABTS (2,2′-Azino-bis-[3-ethylbenzothiazoline-6-sulphonic acid]) 5 mg/ml + 9 ml of substrate buffer (0,1 M Phosphate-Citrate buffer of pH 4 containing 0,03 % H 2 O 2 ), and incubate for 10 minutes at room temperature. Colour development is stopped by adding 100 μl/well of 2 % (w/v) SDS (sodium dodecyl sulphate).

---
Source: https://www.legislation.gov.uk/eudr/2009/156/annex/IV/part/A/division/2/division/2.1/division/2.1.4 (the official version)
Page: https://openlegislation.uk/eu-directive-2009-156/annex-IV/part-A/division-2/division-2.1/division-2.1.4
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.