# Directive 2009/54/EC of the European Parliament and of the Council of 18 June 2009 on the exploitation and marketing of natural mineral waters (Recast) (Text with EEA relevance), Division 1.3: Criteria for microbiological analyses at source

Extent: E+W+S+N.I.

These analyses shall include:
1.3.1. a demonstration of the absence of parasites and pathogenic micro-organisms;
1.3.2. a quantitative determination of the revivable colony count indicative of faecal contamination:
(a) absence of Escherichia coli and other coliforms in 250 ml at 37 °C and 44,5 °C;
(b) absence of faecal streptococci in 250 ml;
(c) absence of sporulated sulphite-reducing anaerobes in 50 ml;
(d) absence of Pseudomonas aeruginosa in 250 ml;
1.3.3. determination of the revivable total colony count per ml of water:
(a) at 20 to 22 °C in 72 hours on agar-agar or an agar-gelatine mixture;
(b) at 37 °C in 24 hours on agar-agar.

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Source: https://www.legislation.gov.uk/eudr/2009/54/annex/I/division/II/division/1.3 (the official version)
Page: https://openlegislation.uk/eu-directive-2009-54/annex-I/division-II/division-1.3
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.