Annex V LIST OF ELEMENTS REFERRED TO IN ARTICLE 23(3)

National legislation in force relevant to the acquisition, husbandry, care and use of animals for scientific purposes.

Ethics in relation to human-animal relationship, intrinsic value of life and arguments for and against the use of animals for scientific purposes.

Basic and appropriate species-specific biology in relation to anatomy, physiological features, breeding, genetics and genetic alteration.

Animal behaviour, husbandry and enrichment.

Species-specific methods of handling and procedures, where appropriate.

Animal health management and hygiene.

Recognition of species-specific distress, pain and suffering of most common laboratory species.

Anaesthesia, pain relieving methods and killing.

Use of humane end-points.

Requirement of replacement, reduction and refinement.

Design of procedures and projects, where appropriate.