# Directive 2010/63/EU of the European Parliament and of the Council of 22 September 2010 on the protection of animals used for scientific purposes (Text with EEA relevance), Division 2: Moderate:

Extent: E+W+S+N.I.

(a) frequent application of test substances which produce moderate clinical effects, and withdrawal of blood samples (> 10 % of circulating volume) in a conscious animal within a few days without volume replacement;
(b) acute dose-range finding studies, chronic toxicity/carcinogenicity tests, with non-lethal end-points;
(c) surgery under general anaesthesia and appropriate analgesia, associated with post surgical pain, suffering or impairment of general condition. Examples include: thoracotomy, craniotomy, laparotomy, orchidectomy, lymphadenectomy, thyroidectomy, orthopaedic surgery with effective stabilisation and wound management, organ transplantation with effective management of rejection, surgical implantation of catheters, or biomedical devices (e.g. telemetry transmitters, minipumps etc.);
(d) models of induction of tumours, or spontaneous tumours, that are expected to cause moderate pain or distress or moderate interference with normal behaviour;
(e) irradiation or chemotherapy with a sublethal dose, or with an otherwise lethal dose but with reconstitution of the immune system. Adverse effects would be expected to be mild or moderate and would be short-lived (< 5 days);
(f) breeding of genetically altered animals which are expected to result in a phenotype with moderate effects;
(g) creation of genetically altered animals through surgical procedures;
(h) use of metabolic cages involving moderate restriction of movement over a prolonged period (up to 5 days);
(i) studies with modified diets that do not meet all of the animals’ nutritional needs and are expected to cause moderate clinical abnormality within the time-scale of the study;
(j) withdrawal of food for 48 hours in adult rats;
(k) evoking escape and avoidance reactions where the animal is unable to escape or avoid the stimulus, and are expected to result in moderate distress.

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Source: https://www.legislation.gov.uk/eudr/2010/63/annex/VIII/section/III/division/2 (the official version)
Page: https://openlegislation.uk/eu-directive-2010-63/annex-VIII/section-III/division-2
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.