# Commission Regulation (EEC) No 2568/91 of 11 July 1991 on the characteristics of olive oil and olive-residue oil and on the relevant methods of analysis, Division 5.2.2: Choice of operating conditions

Extent: E+W+S+N.I.

The operating conditions are generally as follows:
column temperature:
20 °C/minute
5 °C/minute
20 °C/minute
Initially 80 °C
(1′)
→
240 °C
→
325 °C
(6′)
→
340 °C
(10′)
detector temperature: 350 °C;
quantity of substance injected: 1 μl of the n-heptane solution (2-4 ml);
carrier gas: helium or hydrogen at the correct linear velocity for the gas selected (see Appendix);
instrument sensitivity: suitable for the following conditions:
The conditions may be modified according to the characteristics of the column and the GC apparatus to obtain separation of all the waxes and a satisfactory peak resolution (see figure); the internal standard C 32 retention time must be 18 ± 3 minutes. The most representative wax peak must be at least 60 % of the full scale.
The peak integration parameters must be established so as to obtain a correct evaluation of the areas of the peaks in question.

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Source: https://www.legislation.gov.uk/eur/1991/2568/annex/IV/division/5/division/5.2/division/5.2.2 (the official version)
Page: https://openlegislation.uk/eu-regulation-1991-2568/annex-IV/division-5/division-5.2/division-5.2.2
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.