# Commission Regulation (EEC) No 2568/91 of 11 July 1991 on the characteristics of olive oil and olive-residue oil and on the relevant methods of analysis, Division 5.1.3: Preparation of the chromatography column

Extent: E+W+S+N.I.

Pour about 30 ml of the developer solvent (4.10) into the column (3.3), insert a piece of cotton into the bottom part of the column using a glass rod; press to eliminate the air.
In a beaker prepare a suspension of 25 g of silica gel (4.1) in about 80 ml of developer solvent and pour it into the column using a funnel.
Check that all the silica gel is in the column; wash with developer solvent (4.10), open the stopcock and allow the liquid to reach a level about 2 mm above the level of the silica gel.

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Source: https://www.legislation.gov.uk/eur/1991/2568/annex/VII/division/5/division/5.1/division/5.1.3 (the official version)
Page: https://openlegislation.uk/eu-regulation-1991-2568/annex-VII/division-5/division-5.1/division-5.1.3
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.