# Commission Regulation (EEC) No 2568/91 of 11 July 1991 on the characteristics of olive oil and olive-residue oil and on the relevant methods of analysis, Division 5.1.4: Column chromatography

Extent: E+W+S+N.I.

Weigh accurately 1,0 g of sample prepared as in point 5.1 into a 25 ml Erlenmeyer flask (3.1).
Dissolve the sample in 10 ml of developer solvent (4.10). Pour the solution into the chromatography column prepared as in point 5.1.3. Avoid disturbing the surface of the column.
Open the stopcock and pour the sample solution until it reaches the level of the silica gel. Develop with 150 ml of the developer solvent. Adjust the flow rate to 2 ml/min (so that 150 ml enters the column in about 60-70 minutes).
Recover the eluate in a previously weighed 250 ml flask. Evaporate the solvent under vacuum and remove the final traces of the solvent under a nitrogen current.
Weigh the flask and calculate the recovered extract.
(If ready-to-use silica gel SPE cartridges are used use the following method: Put 1 ml of solution (5.1.2) into the prepared cartridges with 3 ml of n-hexane.
After percolating the solution develop with 4 ml of n-hexane/diethyl ether 9/1 (v/v).
Recover the eluate in a 10 ml tube and evaporate to dry in a nitrogen current.
Expose the dry residue to pancreatic lipase (5.2). (It is essential to check the fatty acid composition before and after crossing the SPE cartridge.)

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Source: https://www.legislation.gov.uk/eur/1991/2568/annex/VII/division/5/division/5.1/division/5.1.4 (the official version)
Page: https://openlegislation.uk/eu-regulation-1991-2568/annex-VII/division-5/division-5.1/division-5.1.4
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.