# Commission Regulation (EEC) No 2568/91 of 11 July 1991 on the characteristics of olive oil and olive-residue oil and on the relevant methods of analysis, Division 5.4: Gas chromatography

Extent: E+W+S+N.I.

Operating conditions:
Injector temperature (on-column injector) lower than solvent boiling point (68 °C);
Detector temperature: 350 °C;
Column temperature: programming of furnace temperature: 60 °C for 1 minute, increasing by 15 °C per minute up to 180 °C, then by 5 °C per minute up to 340 °C, then 340 °C for 13 minutes;
Carrier gas: hydrogen or helium, set at a linear velocity sufficient to obtain the resolution reflected in Figure 1. The retention time of the C 54 triglyceride must be 40 ± 5 minutes (see Figure 2). (The operating conditions indicated above are indicative. Operators will have to optimise them to obtain the desired resolution. The peak corresponding to 2-glyceryl monopalmitate must have a minimum height equal to 10 % of the recorder scale.)
Quantity of substance injected: 0,5-1 μl of the n-hexane solution (5 ml) (5.3.3).

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Source: https://www.legislation.gov.uk/eur/1991/2568/annex/VII/division/5/division/5.4 (the official version)
Page: https://openlegislation.uk/eu-regulation-1991-2568/annex-VII/division-5/division-5.4
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.