{
  "url": "https://openlegislation.uk/eu-regulation-1991-2568/annex-XIX/appendix",
  "json_url": "https://openlegislation.uk/eu-regulation-1991-2568/annex-XIX/appendix.json",
  "legislation": "eur/1991/2568",
  "legislation_title": "Commission Regulation (EEC) No 2568/91 of 11 July 1991 on the characteristics of olive oil and olive-residue oil and on the relevant methods of analysis",
  "legislation_url": "https://openlegislation.uk/eu-regulation-1991-2568",
  "path": "annex/XIX/appendix",
  "kind": "appendix",
  "number": null,
  "heading": null,
  "text": "1\nHydrocarbons\n2\nα-Tocopherol\n3\nPrenols\n4\nTriterpenic Alcohols\n5\nAliphatic Alcohols\n6\nMethyl Sterols\n7\nSterols\n8\nTriterpenic Dialcohols\nFigure 1 — TLC of the unsaponifiable fraction from olive pomace oil eluted twice with hexane:diethyl ether (65:35), developed with SO4H2 (50 %) and heated. The bands that should be scrapped are the ones contained within the rectangle, 1 are the bands for aliphatic alcohols and 2 for the sterols and triterpenic dialcohols.\nTable I — Relative retention times for sterols\nPeak\nIdentification\nRelative retention time\nSE 54 column\nSE 52 column\n1\nCholesterol\nΔ-5-cholesten-3β-ol\n0,67\n0,63\n2\nCholestanol\n5α-cholestan-3β -ol\n0,68\n0,64\n3\nBrassicasterol\n[24S]-24-methyl-Δ-5,22-cholestadien-3β -ol\n0,73\n0,71\n*\nErgosterol\n[24S]-24-methyl-Δ-5,7,22 cholestatrien-3β -ol\n0,78\n0,76\n4\n24-methylene-cholesterol\n24-methylene-Δ-5,24-cholestadien-3β -o1\n0,82\n0,80\n5\nCampesterol\n(24R)-24-methyl-Δ-5-cholesten-3β -ol\n0,83\n0,81\n6\nCampestanol\n(24R)-24-methyl-cholestan-3β -ol\n0,85\n0,82\n7\nStigmasterol\n(24S)-24-ethyl-Δ-5,22-cholestadien-3β -ol\n0,88\n0,87\n8\nΔ-7-campesterol\n(24R)-24-methyl-Δ-7-cholesten-3β -ol\n0,93\n0,92\n9\nΔ-5,23-stigmastadienol\n(24R,S)-24-ethyl-Δ-5,23-choIestadien-3β -ol\n0,95\n0,95\n10\nClerosterol\n(24S)-24-ethyl-Δ-5,25-cholestadien-3β -ol\n0,96\n0,96\n11\nß-sitosterol\n(24R)-24-ethyl-Δ-5-cholesten-3β -ol\n1,00\n1,00\n12\nSitostanol\n24-ethyl-cholestan-3β -ol\n1,02\n1,02\n13\nΔ-5-avenasterol\n(24Z)-24-ethylidene-Δ-cholesten-3β -ol\n1,03\n1,03\n14\nΔ-5,24-stigmastadienol\n(24R,S)-24-ethyl-Δ-5,24-cholestadien-3β -ol\n1,08\n1,08\n15\nΔ-7-stigmastenol\n(24R,S)-24-ethyl-Δ-7-cholesten-3β -ol\n1,12\n1,12\n16\nΔ-7-avenasterol\n(24Z)-24-ethylidene-Δ-7-cholesten-3β -ol\n1,16\n1,16\n17\nErythrodiol\n5α-olean-12-en-3β,28-diol\n1,41\n1,41\n18\nUvaol\nΔ12-ursen-3β,28-diol\n1,52\n1,52\nFigure 2 — GC-FID chromatographic profile of the sterol and triterpenic dialcohols from refined olive oil. (1) Cholesterol, (2) α-cholestanol (I.S.), (3) 24-methylencholesterol, (4) campesterol, (5) campestanol, (6) stigmasterol, (7) Δ5,23-stigmastadienol, (8) clerosterol, (9) β-sitosterol, (10) sitostanol, (11) Δ5-avenasterol, (12) Δ5,24-stigmastadienol, (13) Δ7-stigmastenol, (14) Δ7-avenasterol, (15) erythrodiol, (16) uvaol.\nFigure 3 — GC-FID chromatographic profile of the sterol and triterpenic dialcohols from a lampante olive oil. (1) Cholesterol, (2) α-cholestanol, (3) brassicasterol, (4) 24-methylencholesterol, (5) campesterol, (6) campestanol, (7) stigmasterol, (8) Δ7-campesterol, (9) Δ5,23-stigmastadienol, (10) clerosterol, (11) β-sitosterol, (12) sitostanol, (13) Δ5-avenasterol, (14) Δ5,24-stigmastadienol, (15) Δ7-stigmastenol, (16) Δ7-avenasterol, (17) erythrodiol, (18) uvaol.\nFigure 4 — GC-FID chromatographic profile of aliphatic alcohols and triterpenic alcohols of olive oil. (I.S.) C20-ol, (1) C22-ol, (2) C24-ol, (3) C26-ol, (4) C28-ol, (5) triterpenic alcohols.\nFigure 5 — GC-FID chromatographic profile of aliphatic alcohols and triterpenic alcohols of a refined olive oil and a second centrifugation olive oil. (I.S.) C20-ol, (1) C22-ol, (2) C24-ol, (3) C26-ol, (4) C28-ol, (5) triterpenic alcohols.\nFigure 6 — HPLC Chromatogram of an olive oil unsaponifiable separated by HPLC using a UV detector. (1) Aliphatic and triperpenic alcohols; (2) Sterols and triterpenic dialcohols",
  "extent": "E+W+S+N.I.",
  "status": null,
  "version": "current",
  "annotations": [
    {
      "type": "F",
      "label": "F1",
      "text": "Substituted by Commission Implementing Regulation (EU) 2019/1604 of 27 September 2019 amending Regulation (EEC) No 2568/91 on the characteristics of olive oil and olive-residue oil and on the relevant methods of analysis."
    }
  ],
  "outstanding_effects": [],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/eur/1991/2568/annex/XIX/appendix",
    "retrieved_at": "2026-11-01T15:01:50.986318+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}