# Commission Regulation (EEC) No 2568/91 of 11 July 1991 on the characteristics of olive oil and olive-residue oil and on the relevant methods of analysis, Appendix

Extent: E+W+S+N.I.

1
Hydrocarbons
2
α-Tocopherol
3
Prenols
4
Triterpenic Alcohols
5
Aliphatic Alcohols
6
Methyl Sterols
7
Sterols
8
Triterpenic Dialcohols
Figure 1 — TLC of the unsaponifiable fraction from olive pomace oil eluted twice with hexane:diethyl ether (65:35), developed with SO4H2 (50 %) and heated. The bands that should be scrapped are the ones contained within the rectangle, 1 are the bands for aliphatic alcohols and 2 for the sterols and triterpenic dialcohols.
Table I — Relative retention times for sterols
Peak
Identification
Relative retention time
SE 54 column
SE 52 column
1
Cholesterol
Δ-5-cholesten-3β-ol
0,67
0,63
2
Cholestanol
5α-cholestan-3β -ol
0,68
0,64
3
Brassicasterol
[24S]-24-methyl-Δ-5,22-cholestadien-3β -ol
0,73
0,71
*
Ergosterol
[24S]-24-methyl-Δ-5,7,22 cholestatrien-3β -ol
0,78
0,76
4
24-methylene-cholesterol
24-methylene-Δ-5,24-cholestadien-3β -o1
0,82
0,80
5
Campesterol
(24R)-24-methyl-Δ-5-cholesten-3β -ol
0,83
0,81
6
Campestanol
(24R)-24-methyl-cholestan-3β -ol
0,85
0,82
7
Stigmasterol
(24S)-24-ethyl-Δ-5,22-cholestadien-3β -ol
0,88
0,87
8
Δ-7-campesterol
(24R)-24-methyl-Δ-7-cholesten-3β -ol
0,93
0,92
9
Δ-5,23-stigmastadienol
(24R,S)-24-ethyl-Δ-5,23-choIestadien-3β -ol
0,95
0,95
10
Clerosterol
(24S)-24-ethyl-Δ-5,25-cholestadien-3β -ol
0,96
0,96
11
ß-sitosterol
(24R)-24-ethyl-Δ-5-cholesten-3β -ol
1,00
1,00
12
Sitostanol
24-ethyl-cholestan-3β -ol
1,02
1,02
13
Δ-5-avenasterol
(24Z)-24-ethylidene-Δ-cholesten-3β -ol
1,03
1,03
14
Δ-5,24-stigmastadienol
(24R,S)-24-ethyl-Δ-5,24-cholestadien-3β -ol
1,08
1,08
15
Δ-7-stigmastenol
(24R,S)-24-ethyl-Δ-7-cholesten-3β -ol
1,12
1,12
16
Δ-7-avenasterol
(24Z)-24-ethylidene-Δ-7-cholesten-3β -ol
1,16
1,16
17
Erythrodiol
5α-olean-12-en-3β,28-diol
1,41
1,41
18
Uvaol
Δ12-ursen-3β,28-diol
1,52
1,52
Figure 2 — GC-FID chromatographic profile of the sterol and triterpenic dialcohols from refined olive oil. (1) Cholesterol, (2) α-cholestanol (I.S.), (3) 24-methylencholesterol, (4) campesterol, (5) campestanol, (6) stigmasterol, (7) Δ5,23-stigmastadienol, (8) clerosterol, (9) β-sitosterol, (10) sitostanol, (11) Δ5-avenasterol, (12) Δ5,24-stigmastadienol, (13) Δ7-stigmastenol, (14) Δ7-avenasterol, (15) erythrodiol, (16) uvaol.
Figure 3 — GC-FID chromatographic profile of the sterol and triterpenic dialcohols from a lampante olive oil. (1) Cholesterol, (2) α-cholestanol, (3) brassicasterol, (4) 24-methylencholesterol, (5) campesterol, (6) campestanol, (7) stigmasterol, (8) Δ7-campesterol, (9) Δ5,23-stigmastadienol, (10) clerosterol, (11) β-sitosterol, (12) sitostanol, (13) Δ5-avenasterol, (14) Δ5,24-stigmastadienol, (15) Δ7-stigmastenol, (16) Δ7-avenasterol, (17) erythrodiol, (18) uvaol.
Figure 4 — GC-FID chromatographic profile of aliphatic alcohols and triterpenic alcohols of olive oil. (I.S.) C20-ol, (1) C22-ol, (2) C24-ol, (3) C26-ol, (4) C28-ol, (5) triterpenic alcohols.
Figure 5 — GC-FID chromatographic profile of aliphatic alcohols and triterpenic alcohols of a refined olive oil and a second centrifugation olive oil. (I.S.) C20-ol, (1) C22-ol, (2) C24-ol, (3) C26-ol, (4) C28-ol, (5) triterpenic alcohols.
Figure 6 — HPLC Chromatogram of an olive oil unsaponifiable separated by HPLC using a UV detector. (1) Aliphatic and triperpenic alcohols; (2) Sterols and triterpenic dialcohols

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Source: https://www.legislation.gov.uk/eur/1991/2568/annex/XIX/appendix (the official version)
Page: https://openlegislation.uk/eu-regulation-1991-2568/annex-XIX/appendix
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.