# Commission Regulation (EEC) No 2568/91 of 11 July 1991 on the characteristics of olive oil and olive-residue oil and on the relevant methods of analysis, Division 6.5: Quantitative evaluation

Extent: E+W+S+N.I.

The peak areas of 1-eicosanol and of the aliphatic alcohols C22, C24, C26, C28 are calculated by a data acquisition system. The response factor for 1-eicosanol should be considered equal to 1.
Calculate the areas of the α-cholestanol and the sterol and triterpene dialcohols peaks by using the computing system. Ignore peaks for any compound, which are not included (ergosterol must not be calculated) among those listed in Table 1. The response factor for α-cholestanol should be considered equal to 1.
Calculate the concentration of each individual alcoholic compound, in mg/kg of fatty material, as follows:
Alcoholic compound x = A x × m s A s × m × 1000
where:
A x Peak area for alcoholic compound x, in computing system counts.
A s Area of the 1-eicosanol/α-cholestanol peak, in computing system counts.
m s Mass of added 1-eicosanol/α-cholestanol, in milligrams.
m Mass of the sample used for determination, in grams.

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Source: https://www.legislation.gov.uk/eur/1991/2568/annex/XIX/part/3/division/6/division/6.5 (the official version)
Page: https://openlegislation.uk/eu-regulation-1991-2568/annex-XIX/part-3/division-6/division-6.5
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.