# Commission Regulation (EEC) No 2568/91 of 11 July 1991 on the characteristics of olive oil and olive-residue oil and on the relevant methods of analysis, Division 6.3.1

Extent: E+W+S+N.I.

Working conditions for split injection:
injector temperature: 300 °C,
detector temperature: 320 °C,
integrator-recorder: the parameters for integration should be fixed so as to give a correct assessment of the areas. Valley-valley integration mode is recommended,
sensitivity: about 16 times the minimum attenuation,
amount of solution injected: 1μl,
oven programming temperatures: initial 235 °C for six minutes and then rising at 2 °C/minute up to 285 °C,
injector with 1: 15 flow divider,
carrier: helium or hydrogen at about 120 kPa pressure.
These conditions may be adjusted in accordance with the characteristics of the chromatograph and the column to give chromatograms meeting the following requirements: internal standard peak within approximately five minutes of the time given in 6.3.2; the internal standard peak should be at least 80 % of the full scale.
The gas chromatographic system must be checked injecting a mixture of the stock solution of cholestadiene (5.6) and n-nonacosane solution (5.8). The cholesta-3,5-diene peak must appear before the n-nonacosane (Figure 1c); if it does not occur two actions can be undertaken: reduce the oven temperature and/or use a less polar column.

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Source: https://www.legislation.gov.uk/eur/1991/2568/annex/XVII/division/6/division/6.3/division/6.3.1 (the official version)
Page: https://openlegislation.uk/eu-regulation-1991-2568/annex-XVII/division-6/division-6.3/division-6.3.1
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.