# Commission Regulation (EEC) No 2568/91 of 11 July 1991 on the characteristics of olive oil and olive-residue oil and on the relevant methods of analysis, Division 6.3.3

Extent: E+W+S+N.I.

Quantitative analysis
The stigmastadienes content is determined according to the formula:
mg/kg of stigmastadienes = A s × M c A c × M o
where:
A s area of stigmastadienes peak (if the peak is resolved into two isomers, sum of areas of the two peaks),
A c area of internal standard (cholestadiene),
M c mass of standard added, in micrograms,
M o mass of oil taken, in grams.
Detection limit: about 0,01 mg/kg.
Note 10: When stigmastadienes appear in concentrations of more than 4 mg/kg, if quantifying is required, the method of the International Olive Council for determination of sterenes in refined oil must be applied.

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Source: https://www.legislation.gov.uk/eur/1991/2568/annex/XVII/division/6/division/6.3/division/6.3.3 (the official version)
Page: https://openlegislation.uk/eu-regulation-1991-2568/annex-XVII/division-6/division-6.3/division-6.3.3
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.