Division

F1Note 1 : The elution order can be determined by calculating the equivalent carbon numbers, often defined by the relation $\mathrm{ECN}=\mathrm{CN}-\mathrm{2n}$ , where CN is the carbon number and n is the number of double bonds; it can be calculated more precisely by taking into account the origin of the double bond. If n _o , n _l and n _ln are the numbers of double bonds attributed to oleic, linoleic and linolenic acids respectively, the equivalent carbon number can be calculated by means of the relation of the formula:

$\mathrm{EN}=\mathrm{CN}-d_o{n}_o-d_l{n}_l-d_{\ln }{n}_{\ln }$

where the coefficient d _o , d _l and d _ln can be calculated by means of the reference triglycerides. Under the conditions specified in this method, the relation obtained will be close to:

$\mathrm{ECN}=\mathrm{CN}-\left({\mathrm{2,60\; n}}_o\right)-\left({\mathrm{2,35\; n}}_l\right)-\left({\mathrm{2,17\; n}}_{\ln }\right)$