{
  "url": "https://openlegislation.uk/eu-regulation-1991-2568/annex-XVIII/division-4/division-4.3/division-4.3.1",
  "json_url": "https://openlegislation.uk/eu-regulation-1991-2568/annex-XVIII/division-4/division-4.3/division-4.3.1.json",
  "legislation": "eur/1991/2568",
  "legislation_title": "Commission Regulation (EEC) No 2568/91 of 11 July 1991 on the characteristics of olive oil and olive-residue oil and on the relevant methods of analysis",
  "legislation_url": "https://openlegislation.uk/eu-regulation-1991-2568",
  "path": "annex/XVIII/division/4/division/4.3/division/4.3.1",
  "kind": "division",
  "number": "4.3.1",
  "heading": "Chromatographic column preparation",
  "text": "Fill the column (4.1.3) with about 30 ml of elution solvent (4.2.3), then introduce inside the column some glass wool (4.2.5) pushing it to the bottom of the column by means of the glass rod (4.1.5).\nIn a 100 ml beaker, suspend 25 g of silica gel (4.2.4) in 80 ml of elution mixture (4.2.3), then transfer it to the column by means of a glass funnel (4.1.6).\nTo ensure the complete transfer of the silica gel to the column, wash the beaker with the elution mixture and transfer the washing portions to the column too.\nOpen the cock and let the solvent elute from the column until its level is about 1 cm over the silica gel.",
  "extent": "E+W+S+N.I.",
  "status": null,
  "version": "current",
  "annotations": [
    {
      "type": "F",
      "label": "F1",
      "text": "Substituted by Commission Implementing Regulation (EU) No 299/2013 of 26 March 2013 amending Regulation (EEC) No 2568/91 on the characteristics of olive oil and olive-residue oil and on the relevant methods of analysis."
    }
  ],
  "outstanding_effects": [],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/eur/1991/2568/annex/XVIII/division/4/division/4.3/division/4.3.1",
    "retrieved_at": "2026-11-01T15:01:50.986318+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}