# Commission Regulation (EEC) No 2568/91 of 11 July 1991 on the characteristics of olive oil and olive-residue oil and on the relevant methods of analysis, Division 4.3.1: Chromatographic column preparation

Extent: E+W+S+N.I.

Fill the column (4.1.3) with about 30 ml of elution solvent (4.2.3), then introduce inside the column some glass wool (4.2.5) pushing it to the bottom of the column by means of the glass rod (4.1.5).
In a 100 ml beaker, suspend 25 g of silica gel (4.2.4) in 80 ml of elution mixture (4.2.3), then transfer it to the column by means of a glass funnel (4.1.6).
To ensure the complete transfer of the silica gel to the column, wash the beaker with the elution mixture and transfer the washing portions to the column too.
Open the cock and let the solvent elute from the column until its level is about 1 cm over the silica gel.

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Source: https://www.legislation.gov.uk/eur/1991/2568/annex/XVIII/division/4/division/4.3/division/4.3.1 (the official version)
Page: https://openlegislation.uk/eu-regulation-1991-2568/annex-XVIII/division-4/division-4.3/division-4.3.1
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.