# Commission Regulation (EEC) No 2568/91 of 11 July 1991 on the characteristics of olive oil and olive-residue oil and on the relevant methods of analysis, Division 4.3.3: SPE purification

Extent: E+W+S+N.I.

Silica SPE column is activated by passing 6 ml of hexane (4.2.3) under vacuum, avoiding dryness.
Weigh to an accuracy of 0,001 g, 0,12 g in a 2 ml vial (4.1.15) and dissolve with 0,5 ml of hexane (4.2.3).
Load the SPE column with the solution and elute with 10 ml of hexane-diethyl ether (87:13 v/v) (4.2.3) under vacuum.
The collected fraction is evaporated to dryness in a rotary evaporator (4.1.9) under reduced pressure at room temperature. The residue is dissolved in 2 ml of acetone (4.2.6) for triacylglycerol (TAG) analysis.

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Source: https://www.legislation.gov.uk/eur/1991/2568/annex/XVIII/division/4/division/4.3/division/4.3.3 (the official version)
Page: https://openlegislation.uk/eu-regulation-1991-2568/annex-XVIII/division-4/division-4.3/division-4.3.3
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.