# Commission Regulation (EEC) No 2568/91 of 11 July 1991 on the characteristics of olive oil and olive-residue oil and on the relevant methods of analysis, Division 4.2

Extent: E+W+S+N.I.

n-hexane, chromatography grade or residue grade. Hexane may be replaced by iso-octane (2,2,4-trimethyl pentane in chromatography grade), provided that comparable precision values are achieved. Solvents with higher boiling point than n-hexane take longer to evaporate. However, they are preferred due to the toxicity of hexane. The purity must by checked; for example, the residue after evaporation of 100 ml of solvent may be controlled.
WARNING — Fumes may ignite. Keep away from sources of heat, sparks or naked flames. Make sure the bottles are always properly closed. Ensure proper ventilation during usage. Avoid build-up of fumes and remove any possible fire risk, such as heaters or electric apparatus not manufactured from non-inflammable material. Pernicious if inhaled, because it may cause nerve cell damage. Avoid breathing in the fumes. Use a suitable respiratory apparatus if necessary. Avoid contact with eyes and skin.
Iso-octane is a flammable liquid that presents a fire hazard. Explosion limits in air are 1,1 % to 6,0 % (volume fraction). It is toxic by ingestion and inhalation. Use a ventilated hood in good operating condition to work with this solvent.

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Source: https://www.legislation.gov.uk/eur/1991/2568/annex/XX/division/4/division/4.2 (the official version)
Page: https://openlegislation.uk/eu-regulation-1991-2568/annex-XX/division-4/division-4.2
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.