# Commission Regulation (EEC) No 2568/91 of 11 July 1991 on the characteristics of olive oil and olive-residue oil and on the relevant methods of analysis, Division Note 5:: 100 μl of Sudan I dye at 1 % in the elution mixture can be added to the sample solution to check visually that the waxes are eluted properly.

Extent: E+W+S+N.I.

The retention time of the dye lies in between that of the waxes and triacylglycerols. Hence, when the dye reaches the bottom of the chromatography column, elution has to be suspended because all the waxes have been eluted.

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Source: https://www.legislation.gov.uk/eur/1991/2568/annex/XX/division/5/division/5.1/division/2 (the official version)
Page: https://openlegislation.uk/eu-regulation-1991-2568/annex-XX/division-5/division-5.1/division-2
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.