{
  "url": "https://openlegislation.uk/eu-regulation-2000-1353/annex-II",
  "json_url": "https://openlegislation.uk/eu-regulation-2000-1353/annex-II.json",
  "legislation": "eur/2000/1353",
  "legislation_title": "Commission Regulation (EC) No 1353/2000 of 26 June 2000 concerning the permanent authorisation of an additive and the provisional authorisation of new additives, new additive uses and new preparations in feedingstuffs (Text with EEA relevance)",
  "legislation_url": "https://openlegislation.uk/eu-regulation-2000-1353",
  "path": "annex/II",
  "kind": "annex",
  "number": "II",
  "heading": null,
  "text": "1 CU is the amount of enzyme which liberates 0,128 micromoles of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 4,5 and 30 °C.\n1 EPU is the amount of enzyme which liberates 0,0083 micromoles of reducing sugars (xylose equivalents) from oat spelt xylan per minute at pH 4,7 and 30 °C.\nNo(or EC No)\nAdditive\nChemical formula, description\nSpecies or category of animal\nMaximum age\nMinimum content\nMaximum content\nOther provisions\nPeriod of authorisation\nUnits of activity/kg of complete feedingstuff\n16\nEndo-1,4-beta-glucanase\nEC 3.2.1.4\nPreparation of endo-1,4-beta-glucanase produced by Trichoderma longibrachiatum (IMI SD 142) having a minimum activity of:\nSolid form: 2 000 CU/g\nLiquid form: 2 000 CU/ml\nChickens for fattening\n—\n250 CU\n—\n1.\nIn the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.\n2.\nRecommended dose per kg of complete feedingstuff: 500-1 000 CU.\n3.\nFor use in compound feed rich in non-starch polysaccharides (mainly beta-glucans), e.g. containing more than 40 % barley.\n30.9.2000\nLaying hens\n—\n250 CU\n—\n1.\nIn the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.\n2.\nRecommended dose per kg of complete feedingstuff: 500-1 000 CU.\n3.\nFor use in compound feed rich in non-starch polysaccharides (mainly beta-glucans), e.g. containing more than 40 % barley.\n30.9.2000\nPiglets\n4 months\n250 CU\n—\n1.\nIn the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.\n2.\nRecommended dose per kg of complete feedingstuff: 500-1 000 CU.\n3.\nFor use in compound feed rich in non-starch polysaccharides (mainly beta-glucans), e.g. containing more than 40 % barley.\n30.9.2000\nPigs for fattening\n—\n250 CU\n—\n1.\nIn the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.\n2.\nRecommended dose per kg of complete feedingstuff: 500-1 000 CU.\n3.\nFor use in compound feed rich in non-starch polysaccharides (mainly beta-glucans), e.g. containing more than 40 % barley.\n30.9.2000\n17\nEndo-1,4-beta-xylanase\nEC 3.2.1.8\nPreparation of endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (IMI SD 135) having a minimum activity of:\nSolid form: 6 000 EPU/g\nLiquid form: 2 000 EPU/ml\nChickens for fattening\n—\n750 EPU\n—\n1.\nIn the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.\n2.\nRecommended dose per kg of complete feedingstuff: 1 500-3 000 EPU.\n3.\nFor use in compound feed rich in non-starch polysaccharides (mainly arabi-noxylans), e.g. containing more than 40 % wheat.\n30.9.2000\nLaying hens\n—\n750 EPU\n—\n1.\nIn the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.\n2.\nRecommended dose per kg of complete feedingstuff: 1 500-3 000 EPU.\n3.\nFor use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans), e.g. containing more than 40 % wheat.\n30.9.2000\nPiglets\n4 months\n750 EPU\n—\n1.\nIn the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.\n2.\nRecommended dose per kg of complete feedingstuff: 1 500-3 000 EPU.\n3.\nFor use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans), e.g. containing more than 40 % wheat.\n30.9.2000\nPigs for fattening\n—\n750 EPU\n—\n1.\nIn the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.\n2.\nRecommended dose per kg of complete feedingstuff: 1 500-3 000 EPU.\n3.\nFor use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans), e.g. containing more than 40 % wheat.\n30.9.2000",
  "extent": "E+W+S+N.I.",
  "status": null,
  "version": "current",
  "annotations": [
    {
      "type": "footnote",
      "label": "f1",
      "text": "1 CU is the amount of enzyme which liberates 0,128 micromoles of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 4,5 and 30 °C."
    },
    {
      "type": "footnote",
      "label": "f2",
      "text": "1 EPU is the amount of enzyme which liberates 0,0083 micromoles of reducing sugars (xylose equivalents) from oat spelt xylan per minute at pH 4,7 and 30 °C."
    }
  ],
  "outstanding_effects": [],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/eur/2000/1353/annex/II",
    "retrieved_at": "2026-11-01T14:57:21.903408+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}