# Commission Regulation (EC) No 1353/2000 of 26 June 2000 concerning the permanent authorisation of an additive and the provisional authorisation of new additives, new additive uses and new preparations in feedingstuffs (Text with EEA relevance), Annex II

Extent: E+W+S+N.I.

1 CU is the amount of enzyme which liberates 0,128 micromoles of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 4,5 and 30 °C.
1 EPU is the amount of enzyme which liberates 0,0083 micromoles of reducing sugars (xylose equivalents) from oat spelt xylan per minute at pH 4,7 and 30 °C.
No(or EC No)
Additive
Chemical formula, description
Species or category of animal
Maximum age
Minimum content
Maximum content
Other provisions
Period of authorisation
Units of activity/kg of complete feedingstuff
16
Endo-1,4-beta-glucanase
EC 3.2.1.4
Preparation of endo-1,4-beta-glucanase produced by Trichoderma longibrachiatum (IMI SD 142) having a minimum activity of:
Solid form: 2 000 CU/g
Liquid form: 2 000 CU/ml
Chickens for fattening
—
250 CU
—
1.
In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.
2.
Recommended dose per kg of complete feedingstuff: 500-1 000 CU.
3.
For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans), e.g. containing more than 40 % barley.
30.9.2000
Laying hens
—
250 CU
—
1.
In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.
2.
Recommended dose per kg of complete feedingstuff: 500-1 000 CU.
3.
For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans), e.g. containing more than 40 % barley.
30.9.2000
Piglets
4 months
250 CU
—
1.
In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.
2.
Recommended dose per kg of complete feedingstuff: 500-1 000 CU.
3.
For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans), e.g. containing more than 40 % barley.
30.9.2000
Pigs for fattening
—
250 CU
—
1.
In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.
2.
Recommended dose per kg of complete feedingstuff: 500-1 000 CU.
3.
For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans), e.g. containing more than 40 % barley.
30.9.2000
17
Endo-1,4-beta-xylanase
EC 3.2.1.8
Preparation of endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (IMI SD 135) having a minimum activity of:
Solid form: 6 000 EPU/g
Liquid form: 2 000 EPU/ml
Chickens for fattening
—
750 EPU
—
1.
In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.
2.
Recommended dose per kg of complete feedingstuff: 1 500-3 000 EPU.
3.
For use in compound feed rich in non-starch polysaccharides (mainly arabi-noxylans), e.g. containing more than 40 % wheat.
30.9.2000
Laying hens
—
750 EPU
—
1.
In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.
2.
Recommended dose per kg of complete feedingstuff: 1 500-3 000 EPU.
3.
For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans), e.g. containing more than 40 % wheat.
30.9.2000
Piglets
4 months
750 EPU
—
1.
In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.
2.
Recommended dose per kg of complete feedingstuff: 1 500-3 000 EPU.
3.
For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans), e.g. containing more than 40 % wheat.
30.9.2000
Pigs for fattening
—
750 EPU
—
1.
In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.
2.
Recommended dose per kg of complete feedingstuff: 1 500-3 000 EPU.
3.
For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans), e.g. containing more than 40 % wheat.
30.9.2000

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Source: https://www.legislation.gov.uk/eur/2000/1353/annex/II (the official version)
Page: https://openlegislation.uk/eu-regulation-2000-1353/annex-II
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.