{
  "url": "https://openlegislation.uk/eu-regulation-2000-1353/annex-III",
  "json_url": "https://openlegislation.uk/eu-regulation-2000-1353/annex-III.json",
  "legislation": "eur/2000/1353",
  "legislation_title": "Commission Regulation (EC) No 1353/2000 of 26 June 2000 concerning the permanent authorisation of an additive and the provisional authorisation of new additives, new additive uses and new preparations in feedingstuffs (Text with EEA relevance)",
  "legislation_url": "https://openlegislation.uk/eu-regulation-2000-1353",
  "path": "annex/III",
  "kind": "annex",
  "number": "III",
  "heading": null,
  "text": "1 U is the amount of enzyme which liberates 0,1 micromoles of glucose from carboxymethylcellulose per minute at pH 5.0 ande 40 °C.\n1 U is the amount of enzyme which liberates 0,1 micromoles of glucose from barley beta-glucan per minute at pH 5.0 and 40 °C.\n1 U is the amount of enzyme which liberates 0,1 micromoles of glucose from oat spelt xylan per minute at pH 5.0 ande 40 °C.\n1 EPU is the amount of enzyme which liberates 0,0083 micromoles of reducing sugars (xylose equivalents) from oat spelt xylan per minute at pH 4,7 and 30 °C.\n1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from oat spelt xylan per minute at pH 5,3 and 50 °C.\n1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 5,0 and 30 °C.\n1 U is teh amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from oat spelt xylan per minute at pH 5,3 and 50 °C.\n1 U is the amount of enzyme which liberates 1 micromole of glucosidic linkages from water insoluble cross-linked starch polymer per minute at pH 6,5 and 37 °C.\n1 U is the amount of enzyme which liberates 1 microgram of phenolic compound (tyrosine equivalents) from casein substrate per minute at pH 7,5 and 40 °C.\n1 U is the amount of enzyme which liberates 1 micromole of reducing material (galacturonic acid equivalents) from poly D-galacturonic substrate per minute at pH 5,0 and 40 °C.\n1 FYT is the amount of enzyme which liberates 1 micromole of inorganic phosphate per minute from sodium phytate at pH 5.5 and 37 °C.\n1 IU is the amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from birchwood xylan per minute at pH 4,5 and 30 °C.\n1 U is the amount of enzyme which liberates 0,0056 micromoles of reducing sugars (glucose equivalents) from barley-glucan per minute at pH 7.5 and 30 °C.\n1 U is the amount of enzyme which liberates 0,0056 micromoles of reducing sugars (glucose equivalents) from carboxymethylcellulose per minute at pH 7.5 and 30 °C.\n1 U is the amount of enzyme which liberates 1 micromole of glucose from a cross-linked starch polymer per minute at pH 7.4 and 37 °C.\nNo.(or EC No)\nAdditive\nChemical formula, description\nSpecies or category of animal\nMaximum age\nMinimum content\nMaximum content\nOther provisions\nPeriod of authorisation\nUnits of activity/kg of complete feedingstuff\n12\nEndo-1,4-beta-glucanase\nEC 3.2.1.4\nPreparation of endo-1,4-beta-glucanase, endo-1,3 (4)-beta-glucanase and endo-1,4-beta-xylanase produced by Trichoderma viride (FERM BP-4447) having a minimum activity\nEndo-1,4-beta-glucanase: 8 000 U/g\nEndo-1,3(4)-glucanase: 18 000 U/g\nEndo-1,4-beta-xylanase: 26 000 U/g\nTurkeys for fattening\n—\nEndo-1,4-beta-glucanase 800 U\n—\n1.\nIn the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.\n2.\nRecommended dose per kg of complete feedingstuff:\nEndo-1,4-beta-glucanase: 800-1 200 U\nEndo-1,3 (4)-beta-glucanase: 1 800-2 700 U\nEndo-1,4-beta-xylanase: 2 600-3 900 U.\n3.\nFor use in compound feed rich in non-strach polysaccarides (mainly arabinoxylans and beta-glucans), e.g. containing more than 20 % wheat and 20 % barley.\n30.9.2001\nEndo-1,3(4)-beta-glucanase\nEC 3.2.1.6\nEndo-1,3(4)-beta-glucanase: 1 800 U\n—\nEndo-1,4-beta-xilanase\nEC 3.2.1.8\nEndo-1,4-beta-xylanase: 2 600 U\n—\n17\nEndo-1,4-beta-xylanase\nEC 3.2.1.8\nPreparation of endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (IMI SD 135) having a minimum activity of:\nSolid form: 6 000 EPU/g\nLiquid form: 6 000 EPU/ml\nTurkeys for fattening\n—\n750 EPU\n—\n1.\nIn the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.\n2.\nRecommended dose per kg of complete feedingstuff:\n1 500-3 000 EPU.\n3.\nFor use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans), e.g. containing more than 35 % wheat.\n30.9.2001\n42\nEndo-1,4(4)-betaxylanase\nEC 3.2.1.8\nPreparation of endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (IMI SD 135) having a minimum activity of:\nSolid form: 4 000 U/g\nCharacteristic of the authorised preparation:\nendo-1,4-beta-xylanase: 1,99 %\nwheat: 97,7 %\ncalcium propionate: 0,3 %\nlecithin: 0,01 %\nPigs for fattening\n—\n4 000 U\n—\n1.\nIn the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.\n2.\nRecommended dose per kg of complete feedingstuff:\n4 000 U\n3.\nFor use in compound feed rich in non- starch polysaccharides (mainly arabinoxylans), e.g. containing more than 60 % wheat.\n30.9.2001\n49\nEndo-1,3(4)-beta-glucanase\nEC 3.2.1.6\nPreparation of endo-1,3(4)-beta-glucanase producted by Trichoderma longibrachiatum (ATCC 2106), endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (IMI SD 135) alpha-amylase produced by Bacillus amyloliquefaciens (DSM 9554) and polygalacturonase produced by Aspergillus aculeatus (CBS 589.94) having a minimum activity of:\nendo-1,3(4)-beta-glucanase: 150 U/g\nendo-1,4-beta-xylanase: 1 500 U/g\nalfa-amylase: 500 U/g\nbacillolysin: 800 U/g\npolygalacturonase: 50 U/g\nChickens for fattening\n—\nEndo-1,3(4)-beta-glucanase: 150 U\n—\n1.\nIn the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.\n2.\nRecommended dose per kg of complete feedingstuffs:\nendo-1,3(4)-beta-glucanase: 150 U\nendo-1,4-beta-xylanase: 1 500 U\nalpha-amylase: 800 U.\nbacillolysin: 800 U\npolygalacturonase: 50 U\n3.\nFor use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans and betaglucans), e.g. containing more than 30 % wheat.\n30.9.2001\nEndo-1,4-beta-xilanasa\nEC 3.2.1.8\nEndo-1,4-beta-xylanase: 1 500 U\n—\nAlfa-amylase\nEC 3.2.1.1\nAlfa-amylase: 500 U\n—\nBacillolysin\nEC 3.4.24.28\nBacillolysin 800 U\n—\nPolygalacturonase\nC 3.2.1.15\nPolygalacturonase 50 U\n—\nLayinghens\n—\nendo-1,3(4)-beta-glucanase: 150 U\n—\n1.\nIn the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.\n2.\nRecommended dose per kg of complete feedingstuffs:\nendo-1,3(4)-beta-glucanase: 150 U\nendo-1,4-beta-xylanase: 1 500 U\nalpha-amylase: 1 000 U\npolygalacturonase: 25 U.\n3.\nFor use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans and betaglucans), e.g. containing more than 30 % wheat.\n30.9.2001\nendo-1,4-beta-xylanase: 1 500 U\n—\nalpha-amylase: 1 000 U\n—\nbacillolysin: 800 U\n—\npolygalacturonase: 50 U\n—\n50\n6-phytase\nEC 3.1.3.26\nPreparation of 6-phytase produced by Aspergillus oryzae (DSM 11857) having a minimum activity of:\nCoated form: 2 500 FYT/g\nLiquid form: 5 000 FYT/g\nChickens for fattening\n—\n250 FYT\n—\n1.\nIn the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.\n2.\nRecommended dose per kg of complete feedingstuff:\n500-1 000 FYT\n3.\nFor use in compound feed containing more than 0,25 % phytin bound phosphorus.\n30.9.2001\nLaying hens\n—\n250 FYT\n—\n1.\nIn the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.\n2.\nRecommended dose per kg of complete feedingstuff:\n500-1 000 FYT\n3.\nFor use in compound feed containing more than 0,25 % phytin bound phosphorus.\n30.9.2001\nTurkeys for fattening\n—\n250 FYT\n—\n1.\nIn the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.\n2.\nRecommended dose per kg of complete feedingstuff:\n500-1 000 FYT\n3.\nFor use in compound feed containing more than 0,25 % phytin bound phosphorus.\n30.9.2001\nPiglets\n2 months\n500 FYT\n—\n1.\nIn the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.\n2.\nRecommended dose per kg of complete feedingstuff:\n500-1 000 FYT\n3.\nFor use in compound feed containing more than 0,25 % phytin bound phosphorus.\n30.9.2001\nPigs for fattening\n—\n500 FYT\n—\n1.\nIn the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.\n2.\nRecommended dose per kg of complete feedingstuff:\n500-1 000 FYT\n3.\nFor use in compound feed containing more than 0,25 % phytin bound phosphorus.\n30.9.2001\n51\nEndo-1,4-betaxylanase\nEC 3.2.1.8\nPreparation of endo-1,4-beta-xylanase produced by Bacillus subtilis (LMG-S 15136) having a minimum activity of:\n100 IU/g\nChickens for fattening\n—\n10 IU\n—\n1.\nIn the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.\n2.\nRecommended dose per kg of complete feedingstuff:\n10- IU\n3.\nFor use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans), e.g. containing more than 40 % wheat.\n30.9.2001\n52\nEndo-1,3(4)-beta-glucanase:\nEC 3.2.1.6\nPreparation of endo-1,3(4)-beta-glucanasa produced by Aspergillus aculeatus (CBS 589.94), endo-1,4-beta-glucanase produced by Trichoderma longibrachiatum (CBS 592.94) and alpha-amylase produced by Bacillus amyloliquefaciens (DSM 9553), having a minimum activity of:\nLiquid form:\nEndo-1,3 (4)-beta-glucanase: 10 000 U/m\nEndo-1,4-beta-glucanase: 120 000 U/m\nAlpha-amylase: 400 U/ml\nChickens for fattening\n—\nEndo-1,3(4)-beta-glucanase: 1 000 U\n—\n1.\nIn the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.\n2.\nRecommended dose per kg of complete feedingstuff:\nendo-1,3(4)-beta-glucanase: 1 000-2 000 U endo-1,4-beta-glucanase: 12 000-24 000 U\n3.\nFor use in compound feed rich in non starch polysaccharides (mainly arabinoxylans and betaglucans) e.g. containing more than 20 % wheat and 15 % sorghum and 5 % maize.\n30.9.2001\nEndo-1,4-beta-glucanase:\nEC 3.2.1.4\nEndo-1,4-beta-glucanase: 12 000 U\n—\nAlpha-amylase\nEC 3.2.2.1\nAlpha-amylase: 40 U\n—",
  "extent": "E+W+S+N.I.",
  "status": null,
  "version": "current",
  "annotations": [
    {
      "type": "footnote",
      "label": "f1",
      "text": "1 U is the amount of enzyme which liberates 0,1 micromoles of glucose from carboxymethylcellulose per minute at pH 5.0 ande 40 °C."
    },
    {
      "type": "footnote",
      "label": "f2",
      "text": "1 U is the amount of enzyme which liberates 0,1 micromoles of glucose from barley beta-glucan per minute at pH 5.0 and 40 °C."
    },
    {
      "type": "footnote",
      "label": "f3",
      "text": "1 U is the amount of enzyme which liberates 0,1 micromoles of glucose from oat spelt xylan per minute at pH 5.0 ande 40 °C."
    },
    {
      "type": "footnote",
      "label": "f4",
      "text": "1 EPU is the amount of enzyme which liberates 0,0083 micromoles of reducing sugars (xylose equivalents) from oat spelt xylan per minute at pH 4,7 and 30 °C."
    },
    {
      "type": "footnote",
      "label": "f5",
      "text": "1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from oat spelt xylan per minute at pH 5,3 and 50 °C."
    },
    {
      "type": "footnote",
      "label": "f6",
      "text": "1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 5,0 and 30 °C."
    },
    {
      "type": "footnote",
      "label": "f7",
      "text": "1 U is teh amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from oat spelt xylan per minute at pH 5,3 and 50 °C."
    },
    {
      "type": "footnote",
      "label": "f8",
      "text": "1 U is the amount of enzyme which liberates 1 micromole of glucosidic linkages from water insoluble cross-linked starch polymer per minute at pH 6,5 and 37 °C."
    },
    {
      "type": "footnote",
      "label": "f9",
      "text": "1 U is the amount of enzyme which liberates 1 microgram of phenolic compound (tyrosine equivalents) from casein substrate per minute at pH 7,5 and 40 °C."
    },
    {
      "type": "footnote",
      "label": "f10",
      "text": "1 U is the amount of enzyme which liberates 1 micromole of reducing material (galacturonic acid equivalents) from poly D-galacturonic substrate per minute at pH 5,0 and 40 °C."
    },
    {
      "type": "footnote",
      "label": "f11",
      "text": "1 FYT is the amount of enzyme which liberates 1 micromole of inorganic phosphate per minute from sodium phytate at pH 5.5 and 37 °C."
    },
    {
      "type": "footnote",
      "label": "f12",
      "text": "1 IU is the amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from birchwood xylan per minute at pH 4,5 and 30 °C."
    },
    {
      "type": "footnote",
      "label": "f13",
      "text": "1 U is the amount of enzyme which liberates 0,0056 micromoles of reducing sugars (glucose equivalents) from barley-glucan per minute at pH 7.5 and 30 °C."
    },
    {
      "type": "footnote",
      "label": "f14",
      "text": "1 U is the amount of enzyme which liberates 0,0056 micromoles of reducing sugars (glucose equivalents) from carboxymethylcellulose per minute at pH 7.5 and 30 °C."
    },
    {
      "type": "footnote",
      "label": "f15",
      "text": "1 U is the amount of enzyme which liberates 1 micromole of glucose from a cross-linked starch polymer per minute at pH 7.4 and 37 °C."
    }
  ],
  "outstanding_effects": [],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/eur/2000/1353/annex/III",
    "retrieved_at": "2026-11-01T14:57:21.903408+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}