Division 6 Apparatus and equipment

Apparatus capable of measuring the density and alcoholic strength.

Analytical balance, capable of measuring to four decimal places.

A temperature programmed gas chromatograph fitted with a flame ionisation detector and integrator or other data handling system capable of measuring peak areas or peak heights.

Gas chromatographic column(s), capable of separating the analytes such that the minimum resolution between the individual components (other than 2-methylbutan-1-ol and 3-methylbutan-1-ol) is at least 1.3.

Note 2:

The following columns and GC conditions are suitable examples:

1. 1..

   A retention gap 1 m × 0,32 mm i.d. connected to a CP-WAX 57 CB column 50 m × 0,32 mm i.d. 0,2 μm film thickness (stabilised polyethylene glycol) followed by a Carbowax 400 column 50 m × 0,32 mm i.d. 0,2 μm film thickness. (Columns are connected using press-fit connectors.)

   Carrier gas and pressure:	Helium (135 kPa)
   Column temperature:	35 °C for 17 min., 35 to 70 °C at 12 °C/min., hold at 70 °C for 25 min.
   Injector temperature:	150 °C
   Detector temperature:	250 °C
   Injection volume:	1 μl, split 20 to 100:1

2. 2..

   A retention gap 1 m × 0,32 mm i.d. connected to a CP-WAX 57 CB column 50 m × 0,32 mm i.d. 0,2 μm film thickness (stabilised polyethylene glycol). (Retention gap is connected using a press-fit connector.)

   Carrier gas and pressure:	Helium (65 kPa)
   Column temperature:	35 °C for 10 min., 35 to 110 °C at 5 °C/min., 110 to 190 °C at 30 °C/min., hold at 190 °C for 2 min.
   Injector temperature:	260 °C
   Detector temperature:	300 °C
   Injection volume:	1 μl, split 55:1

3. 3..

   A packed column (5 % CW 20M, Carbopak B), 2 m × 2 mm i.d.

   Column temperature:	65 °C for 4 min., 65 to 140 °C at 10 °C/min., hold at 140 °C for 5 min., 140 to 150 °C at 5 °C/min., hold at 150 °C for 3 min.
   Injector temperature:	65 °C
   Detector temperature:	200 °C
   Injection volume:	1 μl