{
  "url": "https://openlegislation.uk/eu-regulation-2000-2870/annex/division-V/division-12/division-12.4/division-12.4.2",
  "json_url": "https://openlegislation.uk/eu-regulation-2000-2870/annex/division-V/division-12/division-12.4/division-12.4.2.json",
  "legislation": "eur/2000/2870",
  "legislation_title": "Commission Regulation (EC) No 2870/2000 of 19 December 2000 laying down Community reference methods for the analysis of spirits drinks",
  "legislation_url": "https://openlegislation.uk/eu-regulation-2000-2870",
  "path": "annex/division/V/division/12/division/12.4/division/12.4.2",
  "kind": "division",
  "number": "12.4.2",
  "heading": "Extraction",
  "text": "Pipette 5 ml of 96 % ethanol (4.1) into a conical flask, weigh into this flask 50 mg of internal standard (4.3), and add 50 ml of the sample. Add 12 g of ammonium sulphate, anhydrous (12.2.1), and 8.6 g of dibasic sodium phosphate, dodecahydrate (12.2.2). Stopper the conical flask.\nShake the flask for at least 30 minutes. A mechanical shaking device may be used, but not a Teflon coated magnetic stirring bar, as the Teflon will absorb some of the analyte. Note that the added salts will not dissolve completely.\nPlace the stoppered flask in a refrigerator (T < 5 C) for at least two hours.\nAfter this time, there should be two distinct liquid layers and a solid residue. The alcohol layer should be clear; if not, replace in the refrigerator until a clear separation is achieved.\nWhen the alcohol layer is clear, carefully take an aliquot (e.g. 10 ml), without disturbing the aqueous layer, place in an amber vial and close securely.",
  "extent": "E+W+S+N.I.",
  "status": null,
  "version": "current",
  "annotations": [
    {
      "type": "F",
      "label": "F1",
      "text": "Inserted by Commission Regulation (EC) No 2091/2002 of 26 November 2002 amending Regulation (EC) No 2870/2000 laying down Community reference methods for the analysis of spirits drinks."
    }
  ],
  "outstanding_effects": [],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/eur/2000/2870/annex/division/V/division/12/division/12.4/division/12.4.2",
    "retrieved_at": "2026-11-01T14:57:07.594471+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}