# Commission Regulation (EC) No 2870/2000 of 19 December 2000 laying down Community reference methods for the analysis of spirits drinks, Division 4.5.2: Internal standard solution B

Extent: E+W+S+N.I.

Stock solution of internal standard, e.g. estragole (concentration: 2 g/l)
Weigh 40 mg of estragole (4.3) in a 20 ml volumetric flask (400 mg in 200 ml etc.). Add some 96 % vol. ethanol (4.1) make up to volume with 45 % vol. ethanol (4.4), mix thoroughly.

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Source: https://www.legislation.gov.uk/eur/2000/2870/annex/division/V/division/4/division/4.5/division/4.5.2 (the official version)
Page: https://openlegislation.uk/eu-regulation-2000-2870/annex/division-V/division-4/division-4.5/division-4.5.2
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.