# Commission Regulation (EC) No 2870/2000 of 19 December 2000 laying down Community reference methods for the analysis of spirits drinks, Division 4.7.2: Standard solutions used to check the linearity of the response of the instrumentation

Extent: E+W+S+N.I.

A 1,0 g/l stock solution is prepared by weighing, to the nearest 0,1 mg, 100 mg of ammonium glycyrrhizinate in a 100 ml volumetric flask. Add some 50 % vol. ethanol (4.5) and dissolve the ammonium glycyrrhizinate. When it has dissolved make up to the mark with 50 % vol. ethanol (4.5).
At least four other solutions corresponding to 0,05, 0,1, 0,25 and 0,5 g/l of ammonium glycyrrhizinate are prepared by pipetting respectively 5 ml, 10 ml, 25 ml and 50 ml of the 1,0 g/l stock solution in separate 100 ml volumetric flasks. Then make up to the mark with 50 % vol. ethanol (4.5) and mix up thoroughly.
Filter all solutions though a filter for organic solvents.

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Source: https://www.legislation.gov.uk/eur/2000/2870/annex/division/VI/division/4/division/4.7/division/4.7.2 (the official version)
Page: https://openlegislation.uk/eu-regulation-2000-2870/annex/division-VI/division-4/division-4.7/division-4.7.2
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.