{
  "url": "https://openlegislation.uk/eu-regulation-2000-2870/annex/division-VI/division-7/division-7.2",
  "json_url": "https://openlegislation.uk/eu-regulation-2000-2870/annex/division-VI/division-7/division-7.2.json",
  "legislation": "eur/2000/2870",
  "legislation_title": "Commission Regulation (EC) No 2870/2000 of 19 December 2000 laying down Community reference methods for the analysis of spirits drinks",
  "legislation_url": "https://openlegislation.uk/eu-regulation-2000-2870",
  "path": "annex/division/VI/division/7/division/7.2",
  "kind": "division",
  "number": "7.2",
  "heading": "Determination",
  "text": "Once the chromatography conditions have stabilised,\ninject 20 μl of the reference solution C (4.7.1),\ninject 20 μl of the sample solution,\ncompare the two chromatograms. Identify the glycyrrhizic acid peaks from their retention times. Measure their areas (or heights) and calculate the concentration in g/l to two decimal figures using the following equation:\nwhere:\nc is the concentration in grams per litre of glycyrrhizic acid in the spirit being analysed\nC is the concentration in grams per litre of ammonium glycyrrhizinate in the reference solution\nh is the area (or height) of the glycyrrhizic acid peak of the spirit being analysed\nH is the area (or height) of the glycyrrhizic acid peak of the reference solution\nP is the purity of the reference ammonium glycyrrhizinate (in %)\n823 is the mass of one mole of glycyrrhizic acid\n840 is the mass of one mole of ammonium glycyrrhizinate.",
  "extent": "E+W+S+N.I.",
  "status": null,
  "version": "current",
  "annotations": [
    {
      "type": "F",
      "label": "F1",
      "text": "Inserted by Commission Regulation (EC) No 2091/2002 of 26 November 2002 amending Regulation (EC) No 2870/2000 laying down Community reference methods for the analysis of spirits drinks."
    }
  ],
  "outstanding_effects": [],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/eur/2000/2870/annex/division/VI/division/7/division/7.2",
    "retrieved_at": "2026-11-01T14:57:07.594471+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}