# Commission Regulation (EC) No 2870/2000 of 19 December 2000 laying down Community reference methods for the analysis of spirits drinks, Division 7.2: Determination

Extent: E+W+S+N.I.

Once the chromatography conditions have stabilised,
inject 20 μl of the reference solution C (4.7.1),
inject 20 μl of the sample solution,
compare the two chromatograms. Identify the glycyrrhizic acid peaks from their retention times. Measure their areas (or heights) and calculate the concentration in g/l to two decimal figures using the following equation:
where:
c is the concentration in grams per litre of glycyrrhizic acid in the spirit being analysed
C is the concentration in grams per litre of ammonium glycyrrhizinate in the reference solution
h is the area (or height) of the glycyrrhizic acid peak of the spirit being analysed
H is the area (or height) of the glycyrrhizic acid peak of the reference solution
P is the purity of the reference ammonium glycyrrhizinate (in %)
823 is the mass of one mole of glycyrrhizic acid
840 is the mass of one mole of ammonium glycyrrhizinate.

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Source: https://www.legislation.gov.uk/eur/2000/2870/annex/division/VI/division/7/division/7.2 (the official version)
Page: https://openlegislation.uk/eu-regulation-2000-2870/annex/division-VI/division-7/division-7.2
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.