Division 6.5.1 Elution characteristics of solvents A (4.6.2) and B (4.6.3):F1

shift from 20/80 (v/v) to 50/50 (v/v) gradient in 15 minutes,

shift from 50/50 (v/v) to 75/25 (v/v) gradient in five minutes,

equal strength at 75/25 (v/v) for five minutes,

stabilisation of the column between injections,

equal strength at 20/80 (v/v) for five minutes.