# Commission Regulation (EC) No 2870/2000 of 19 December 2000 laying down Community reference methods for the analysis of spirits drinks, Division 5.1

Extent: E+W+S+N.I.

A high-performance liquid chromatograph capable of functioning in binary gradient mode and equipped with:
5.1.1. A spectrophotometric detector capable of measuring at wavelengths from 260 to 340 nm. It is however preferable to work with a multiple wavelength detector with a diode array or similar, in order to confirm the purity of the peaks.
5.1.2. A spectrofluorimetric detector — excitation wavelength: 354 nm, emission wavelength: 446 nm (for the trace determination of scopoletin; which is also detectable at 313 nm by spectrophotometry).
5.1.3. An injection device capable of introducing 10 or 20 μl (for example) of the test sample.
5.1.4. A high-performance liquid chromatography column, RP C18 type, 5 μm maximum particle size.

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Source: https://www.legislation.gov.uk/eur/2000/2870/annex/division/X/division/5/division/5.1 (the official version)
Page: https://openlegislation.uk/eu-regulation-2000-2870/annex/division-X/division-5/division-5.1
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.