{
  "url": "https://openlegislation.uk/eu-regulation-2001-418/annex-II",
  "json_url": "https://openlegislation.uk/eu-regulation-2001-418/annex-II.json",
  "legislation": "eur/2001/418",
  "legislation_title": "Commission Regulation (EC) No 418/2001 of 1 March 2001 concerning the authorisations of new additives and uses of additives in feedingstuffs (Text with EEA relevance)",
  "legislation_url": "https://openlegislation.uk/eu-regulation-2001-418",
  "path": "annex/II",
  "kind": "annex",
  "number": "II",
  "heading": null,
  "text": "1 IFP is the amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from oat xylan per minute at pH 4,8 and 50°C.\n1 BXU is the amount of enzyme which liberates 0,06 micromoles of reducing sugars (xylose equivalents) from birch xylan per minute at pH 5,3 and 50 °C.\n1 BU is the amount of enzyme which liberates 0,06 micromoles of reducing sugars (glucose equivalents) from barley-glucan per minute at pH 4,8 and 50 °C.\n1 PPU is the amount of enzyme which liberates 1 micromole of inorganic phosphate from sodium phytate per minute at pH 5,0 and 37 °C.\n1 U is the amount of enzyme which liberates 5,55 micromoles of reducing sugars (maltose equivalents) from barley beta-glucan per minute at pH 5,0 and 50 °C.\n1 U is the amount of enzyme which liberates 4,00 micromoles of reducing sugars (maltose equivalents) from birchwood xylan per minute at pH 5,5 and 50 °C.\n1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from oat spelt xylan per minute at pH 5,3 and 50 °C.\n1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 5,0 and 30 °C.\n1 U is the amount of enzyme which liberates 1 micromole of phenolic compound (tyrosine equivalents) from a casein substrate per minute at pH 7,5 and 40 °C.\n1 U is the amount of enzyme which liberates 1 micromole of glucosidic linkages from a water insoluble cross-linked starch polymer substrate per minute at pH 6,5 and 37 °C.\n1 U is the amount of enzyme which liberates 1 micromole of reducing material (galacturonic acid equivalents) from a poly D-galacturonic substrate per minute at pH 5,0 and 40 °C.\nNo (or EC No)\nAdditive\nChemical formula, decription\nSpecies or category of animal\nMaximum age\nMinimum content\nMaximum content\nOther provisions\nPeriod of authorisation\nUnits of activity/kg complete feedingstuff\nUnits of activity/kg complete feedingstuff\n23\nEndo-1,4-beta-xylanase\nEC 3.2.1.8\nPreparation of endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (CNCM MA 6-10W) having a minimum of activity of:\nSolid form: 70 000 IFP /g\nLiquid form: 7 000 IFP/ml\nTurkeys for fattening\n—\n700 IFP\n—\n1.\nIn the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting\n2.\nRecommended dose per kilogram of complete feedingstuff: 1 400 IFP\n3.\nFor use in compound feed rich in non- starch polysaccharides (mainly arabinoxylans), e.g. containing more than 40 % wheat\n28.2.2005\nLaying hens\n—\n840 IFP\n—\n1.\nIn the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting\n2.\nRecommended dose per kilogram of complete feedingstuff: 840 IFP\n3.\nFor use in compound feed rich in non- starch polysaccharides (mainly arabinoxylans), e.g. containing more than 40 % wheat\n28.2.2005\n27\nEndo-1,4-beta-xylanase\nEC 3.2.1.8\nEndo-1,3(4)-beta-glucanase\nEC 3.2.1.6\nPreparation of endo-1,4-beta-xylanase produced by Trichoderma reesei (CBS 529.94) and endo-1,3(4)-beta-glucanase produced by Trichoderma reesei (CBS 526.94) having a minimum activity of:\nSolid form:\n200 000 BXU /g\n200 000 BU /g\nLiquid form:\n30 000 BXU/g\n30 000 BU/g\nPiglets\n2 months\n7 500 BXU\n—\n1.\nIn the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting\n2.\nRecommended dose per kilogram of complete feedingstuff:\n7 500 to 15 000 BXU\n7 500 to 15 000 BU\n3.\nFor use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans), e.g. containing more than 50 % wheat\n28.2.2005\n7 500 BU\n—\n28\n3-Phytase\nEC 3.1.3.8\nPreparation of 3-phytase produced by Trichoderma reesei (CBS 528.94) having a minimum activity of\nSolid form: 5 000 PPU /g\nLiquid form: 1 000 PPU/g\nChickens for fattening\n—\n500 PPU\n—\n1.\nIn the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting\n2.\nRecommended dose per kilogram of complete feedingstuff: 500 to 750 PPU\n3.\nFor use in compound feed containing more than 0,22 % phytin bound phosphorus\n28.2.2005\n30\nEndo-1,3(4)-beta-glucanase\nEC 3.2.1.6\nEndo-1,-beta-xylanase\nEC 3.2.1.8\nPreparation of endo-1,3(4)-beta-glucanase and endo-1,4-beta-xylanase produced by Penicillium funiculosum (IMI SD 101) having a minimum activity of:\nPowder form:\nEndo-1,3(4)-beta-glucanase: 2 000 U /g\nEndo-1,4-beta-xylanase: 1 400 U/g /g\nLiquid form:\nEndo-1,3(4)-beta-glucanase: 500 U/ml\nEndo-1,4-beta-xylanase: 350 U/ml\nTurkeys for fattening\n—\nEndo-1,3(4)-beta-glucanase:\n100 U\n—\n1.\nIn the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting\n2.\nRecommended dose per kilogram of complete feedingstuff:\nendo-1,3(4)-beta-glucanase: 100 U\nendo-1,4-beta-xylanase: 70 U\n3.\nFor use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans), e.g. containing more than 50 % wheat\n28.2.2005\nEndo-1,4-beta-xylanase:\n70 U\n—\nLaying hens\n—\nEndo-1,3(4)-beta-glucanase:\n100 U\n—\n1.\nIn the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stabiltiy to pelleting\n2.\nRecommended dose per kilogram of complete feedingstuff:\nendo-1,3(4)-beta-glucanase: 100 U\nendo-1,4-beta-xylanase: 70 U\n3.\nFor use in compound feed rich in non-starch polysaccharides (mainly beta- glucans and arabinoxylans), e.g. continaing more than 60 % barley or 30 % wheat\n28.2.2005\nEndo-1,4-beta-xylanase:\n70 U\n—\nPigs for fattening\n—\nEndo-1,3(4)-beta-glucanase:\n100 U\n—\n1.\nIn the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.\n2.\nRecommended dose per kilogram of complete feedingstuff:\nendo-1,3(4)-beta-glucanase: 100 U\nendo-1,4-beta-xylanase: 70 U\n3.\nFor use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans), e.g. containing more than 50 % barley or 60 % wheat\n28.2.2005\nEndo-1,4-beta-xylanase:\n70 U\n—\n59\nEndo-1,4-beta-xylanase\nEC 3.2.1.8\nPreparation of endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (ATCC 2105), endo-1,3(4)-beta-glucanase and alpha-amylase produced by Bacillus amyloliquefaciens (DSM 9553), subtilisin produced by Bacillus subtilis (ATCC 2107), polygalacturonase produced by Aspergillus aculeatus (CBS 589.94) having a minimum activity of:\nEndo-1,4-beta-xylanase: 300 U /g\nEndo-1,3(4)-beta-glucanase: 150 U /g\nSubtilisin: 4 000 U /g\nAlpha-amylase: 400 U /g\nPolygalacturonase: 25 U /g\nChickens for fattening\n—\nEndo-1,4-beta-xylanase:\n300 U\n—\n1.\nIn the directions for use of the additive and premixture, indicate the storage temperature, storage life and stbility to pelleting\n2.\nRecommended dose per kilogram of complete feedingstuff:\nendo-1,4-beta-xylanase: 300 U\nendo-1,3(4)-beta-glucanase: 150 U\nsubtilisin: 4 000 U\nalpha-amylase: 400 U\npolygalacturonase: 25 U\n3.\nFor use in compound feed rich in starch and non-starch polysaccharides (mainly arabinoxylans and beta-glucans), e.g. containing more than 40 % maize\n28.2.2005\nEndo-1,3(4)-beta-glucanase\nEC 3.2.1.6\nEndo-1,3(4)-beta-glucanase:\n150 U\n—\nSubtilisin\nEC 3.4.21.62\nSubtilisin:\n4 000 U\n—\nAlpha-amylase\nEC 3.2.1.1\nAlpha-amylase:\n400 U\n—\nPolygalacturonase\nEC 3.2.1.15\nPolygalacturonase:\n25 U\n—\n60\nEndo-1,4-beta-xylanase\nEC 3.2.1.8\nPreparation of endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (ATCC 2105), endo-1,3(4)-beta-glucanase produced by Trichoderma longibrachiatum (ATCC 2106) having a minimum activitiy of:\nEndo-1,4-beta-xylanase: 5 000 U /g\nEndo-1,3(4)-beta-glucanase: 50 U /g\nChickens for fattening\n—\nEndo-1,4-beta-xylanase:\n500 U\n—\n1.\nIn the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.\n2.\nRecommended dose per kilogram of complete feedingstuff:\nendo-1,4-beta-xylanase: 500 to 2 500 U\nendo-1,3(4)-beta-glucanase: 5 to 25 U\n3.\nFor use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans), e.g. containing more than 20 % barley and 40 % wheat\n28.2.2005\nEndo-1,3(4)-beta-glucanase\nEC 3.2.1.6\nEndo-1,3(4)-beta-glucanase:\n5 U\n—\n61\nEndo-1,4-beta-xylanase\nEC 3.2.1.8\nPreparation of endo-1,4-beta-xylanase produced by Trichoderma reesei (CBS 526.94) having a minimum activity of:\nPowder form:\nEndo-1,(4)-beta-xylanase: 17 000 BXU /g\nEndo-1,3(4)-beta-xylanase: 11 000 BU/ /g\nLiquid form:\nEndo-1,4-beta-xylanase: 22 000 U/ml\nEndo-1,3(4)-beta-glucanase: 15 000 U/ml\nChickens for fattening\n—\nEndo-1,4-beta-xylanase:\n17 000 BXU\n—\n1.\nIn the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting\n2.\nRecommended dose per kilogram of complete feedingstuff:\nendo-1,4-beta-xylanase: 17 000 BXU\nendo-1,3(4)-beta-glucanase: 11 000 BU\n3.\nFor use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans), e.g. containing more than 40 % barley or 55 % wheat\n28.2.2005\nEndo-1,3(4)-beta-glucanase\nEC 3.2.1.6\nEndo-1,3(4)-beta-glucanase:\n11 000 BU\n—",
  "extent": "E+W+S+N.I.",
  "status": null,
  "version": "current",
  "annotations": [
    {
      "type": "footnote",
      "label": "f1",
      "text": "1 IFP is the amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from oat xylan per minute at pH 4,8 and 50°C."
    },
    {
      "type": "footnote",
      "label": "f2",
      "text": "1 BXU is the amount of enzyme which liberates 0,06 micromoles of reducing sugars (xylose equivalents) from birch xylan per minute at pH 5,3 and 50 °C."
    },
    {
      "type": "footnote",
      "label": "f3",
      "text": "1 BU is the amount of enzyme which liberates 0,06 micromoles of reducing sugars (glucose equivalents) from barley-glucan per minute at pH 4,8 and 50 °C."
    },
    {
      "type": "footnote",
      "label": "f4",
      "text": "1 PPU is the amount of enzyme which liberates 1 micromole of inorganic phosphate from sodium phytate per minute at pH 5,0 and 37 °C."
    },
    {
      "type": "footnote",
      "label": "f5",
      "text": "1 U is the amount of enzyme which liberates 5,55 micromoles of reducing sugars (maltose equivalents) from barley beta-glucan per minute at pH 5,0 and 50 °C."
    },
    {
      "type": "footnote",
      "label": "f6",
      "text": "1 U is the amount of enzyme which liberates 4,00 micromoles of reducing sugars (maltose equivalents) from birchwood xylan per minute at pH 5,5 and 50 °C."
    },
    {
      "type": "footnote",
      "label": "f7",
      "text": "1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from oat spelt xylan per minute at pH 5,3 and 50 °C."
    },
    {
      "type": "footnote",
      "label": "f8",
      "text": "1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 5,0 and 30 °C."
    },
    {
      "type": "footnote",
      "label": "f9",
      "text": "1 U is the amount of enzyme which liberates 1 micromole of phenolic compound (tyrosine equivalents) from a casein substrate per minute at pH 7,5 and 40 °C."
    },
    {
      "type": "footnote",
      "label": "f10",
      "text": "1 U is the amount of enzyme which liberates 1 micromole of glucosidic linkages from a water insoluble cross-linked starch polymer substrate per minute at pH 6,5 and 37 °C."
    },
    {
      "type": "footnote",
      "label": "f11",
      "text": "1 U is the amount of enzyme which liberates 1 micromole of reducing material (galacturonic acid equivalents) from a poly D-galacturonic substrate per minute at pH 5,0 and 40 °C."
    }
  ],
  "outstanding_effects": [],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/eur/2001/418/annex/II",
    "retrieved_at": "2026-11-01T14:57:02.672909+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}