# Commission Regulation (EC) No 418/2001 of 1 March 2001 concerning the authorisations of new additives and uses of additives in feedingstuffs (Text with EEA relevance), Annex II

Extent: E+W+S+N.I.

1 IFP is the amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from oat xylan per minute at pH 4,8 and 50°C.
1 BXU is the amount of enzyme which liberates 0,06 micromoles of reducing sugars (xylose equivalents) from birch xylan per minute at pH 5,3 and 50 °C.
1 BU is the amount of enzyme which liberates 0,06 micromoles of reducing sugars (glucose equivalents) from barley-glucan per minute at pH 4,8 and 50 °C.
1 PPU is the amount of enzyme which liberates 1 micromole of inorganic phosphate from sodium phytate per minute at pH 5,0 and 37 °C.
1 U is the amount of enzyme which liberates 5,55 micromoles of reducing sugars (maltose equivalents) from barley beta-glucan per minute at pH 5,0 and 50 °C.
1 U is the amount of enzyme which liberates 4,00 micromoles of reducing sugars (maltose equivalents) from birchwood xylan per minute at pH 5,5 and 50 °C.
1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from oat spelt xylan per minute at pH 5,3 and 50 °C.
1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 5,0 and 30 °C.
1 U is the amount of enzyme which liberates 1 micromole of phenolic compound (tyrosine equivalents) from a casein substrate per minute at pH 7,5 and 40 °C.
1 U is the amount of enzyme which liberates 1 micromole of glucosidic linkages from a water insoluble cross-linked starch polymer substrate per minute at pH 6,5 and 37 °C.
1 U is the amount of enzyme which liberates 1 micromole of reducing material (galacturonic acid equivalents) from a poly D-galacturonic substrate per minute at pH 5,0 and 40 °C.
No (or EC No)
Additive
Chemical formula, decription
Species or category of animal
Maximum age
Minimum content
Maximum content
Other provisions
Period of authorisation
Units of activity/kg complete feedingstuff
Units of activity/kg complete feedingstuff
23
Endo-1,4-beta-xylanase
EC 3.2.1.8
Preparation of endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (CNCM MA 6-10W) having a minimum of activity of:
Solid form: 70 000 IFP /g
Liquid form: 7 000 IFP/ml
Turkeys for fattening
—
700 IFP
—
1.
In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting
2.
Recommended dose per kilogram of complete feedingstuff: 1 400 IFP
3.
For use in compound feed rich in non- starch polysaccharides (mainly arabinoxylans), e.g. containing more than 40 % wheat
28.2.2005
Laying hens
—
840 IFP
—
1.
In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting
2.
Recommended dose per kilogram of complete feedingstuff: 840 IFP
3.
For use in compound feed rich in non- starch polysaccharides (mainly arabinoxylans), e.g. containing more than 40 % wheat
28.2.2005
27
Endo-1,4-beta-xylanase
EC 3.2.1.8
Endo-1,3(4)-beta-glucanase
EC 3.2.1.6
Preparation of endo-1,4-beta-xylanase produced by Trichoderma reesei (CBS 529.94) and endo-1,3(4)-beta-glucanase produced by Trichoderma reesei (CBS 526.94) having a minimum activity of:
Solid form:
200 000 BXU /g
200 000 BU /g
Liquid form:
30 000 BXU/g
30 000 BU/g
Piglets
2 months
7 500 BXU
—
1.
In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting
2.
Recommended dose per kilogram of complete feedingstuff:
7 500 to 15 000 BXU
7 500 to 15 000 BU
3.
For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans), e.g. containing more than 50 % wheat
28.2.2005
7 500 BU
—
28
3-Phytase
EC 3.1.3.8
Preparation of 3-phytase produced by Trichoderma reesei (CBS 528.94) having a minimum activity of
Solid form: 5 000 PPU /g
Liquid form: 1 000 PPU/g
Chickens for fattening
—
500 PPU
—
1.
In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting
2.
Recommended dose per kilogram of complete feedingstuff: 500 to 750 PPU
3.
For use in compound feed containing more than 0,22 % phytin bound phosphorus
28.2.2005
30
Endo-1,3(4)-beta-glucanase
EC 3.2.1.6
Endo-1,-beta-xylanase
EC 3.2.1.8
Preparation of endo-1,3(4)-beta-glucanase and endo-1,4-beta-xylanase produced by Penicillium funiculosum (IMI SD 101) having a minimum activity of:
Powder form:
Endo-1,3(4)-beta-glucanase: 2 000 U /g
Endo-1,4-beta-xylanase: 1 400 U/g /g
Liquid form:
Endo-1,3(4)-beta-glucanase: 500 U/ml
Endo-1,4-beta-xylanase: 350 U/ml
Turkeys for fattening
—
Endo-1,3(4)-beta-glucanase:
100 U
—
1.
In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting
2.
Recommended dose per kilogram of complete feedingstuff:
endo-1,3(4)-beta-glucanase: 100 U
endo-1,4-beta-xylanase: 70 U
3.
For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans), e.g. containing more than 50 % wheat
28.2.2005
Endo-1,4-beta-xylanase:
70 U
—
Laying hens
—
Endo-1,3(4)-beta-glucanase:
100 U
—
1.
In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stabiltiy to pelleting
2.
Recommended dose per kilogram of complete feedingstuff:
endo-1,3(4)-beta-glucanase: 100 U
endo-1,4-beta-xylanase: 70 U
3.
For use in compound feed rich in non-starch polysaccharides (mainly beta- glucans and arabinoxylans), e.g. continaing more than 60 % barley or 30 % wheat
28.2.2005
Endo-1,4-beta-xylanase:
70 U
—
Pigs for fattening
—
Endo-1,3(4)-beta-glucanase:
100 U
—
1.
In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.
2.
Recommended dose per kilogram of complete feedingstuff:
endo-1,3(4)-beta-glucanase: 100 U
endo-1,4-beta-xylanase: 70 U
3.
For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans), e.g. containing more than 50 % barley or 60 % wheat
28.2.2005
Endo-1,4-beta-xylanase:
70 U
—
59
Endo-1,4-beta-xylanase
EC 3.2.1.8
Preparation of endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (ATCC 2105), endo-1,3(4)-beta-glucanase and alpha-amylase produced by Bacillus amyloliquefaciens (DSM 9553), subtilisin produced by Bacillus subtilis (ATCC 2107), polygalacturonase produced by Aspergillus aculeatus (CBS 589.94) having a minimum activity of:
Endo-1,4-beta-xylanase: 300 U /g
Endo-1,3(4)-beta-glucanase: 150 U /g
Subtilisin: 4 000 U /g
Alpha-amylase: 400 U /g
Polygalacturonase: 25 U /g
Chickens for fattening
—
Endo-1,4-beta-xylanase:
300 U
—
1.
In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stbility to pelleting
2.
Recommended dose per kilogram of complete feedingstuff:
endo-1,4-beta-xylanase: 300 U
endo-1,3(4)-beta-glucanase: 150 U
subtilisin: 4 000 U
alpha-amylase: 400 U
polygalacturonase: 25 U
3.
For use in compound feed rich in starch and non-starch polysaccharides (mainly arabinoxylans and beta-glucans), e.g. containing more than 40 % maize
28.2.2005
Endo-1,3(4)-beta-glucanase
EC 3.2.1.6
Endo-1,3(4)-beta-glucanase:
150 U
—
Subtilisin
EC 3.4.21.62
Subtilisin:
4 000 U
—
Alpha-amylase
EC 3.2.1.1
Alpha-amylase:
400 U
—
Polygalacturonase
EC 3.2.1.15
Polygalacturonase:
25 U
—
60
Endo-1,4-beta-xylanase
EC 3.2.1.8
Preparation of endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (ATCC 2105), endo-1,3(4)-beta-glucanase produced by Trichoderma longibrachiatum (ATCC 2106) having a minimum activitiy of:
Endo-1,4-beta-xylanase: 5 000 U /g
Endo-1,3(4)-beta-glucanase: 50 U /g
Chickens for fattening
—
Endo-1,4-beta-xylanase:
500 U
—
1.
In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.
2.
Recommended dose per kilogram of complete feedingstuff:
endo-1,4-beta-xylanase: 500 to 2 500 U
endo-1,3(4)-beta-glucanase: 5 to 25 U
3.
For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans), e.g. containing more than 20 % barley and 40 % wheat
28.2.2005
Endo-1,3(4)-beta-glucanase
EC 3.2.1.6
Endo-1,3(4)-beta-glucanase:
5 U
—
61
Endo-1,4-beta-xylanase
EC 3.2.1.8
Preparation of endo-1,4-beta-xylanase produced by Trichoderma reesei (CBS 526.94) having a minimum activity of:
Powder form:
Endo-1,(4)-beta-xylanase: 17 000 BXU /g
Endo-1,3(4)-beta-xylanase: 11 000 BU/ /g
Liquid form:
Endo-1,4-beta-xylanase: 22 000 U/ml
Endo-1,3(4)-beta-glucanase: 15 000 U/ml
Chickens for fattening
—
Endo-1,4-beta-xylanase:
17 000 BXU
—
1.
In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting
2.
Recommended dose per kilogram of complete feedingstuff:
endo-1,4-beta-xylanase: 17 000 BXU
endo-1,3(4)-beta-glucanase: 11 000 BU
3.
For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans), e.g. containing more than 40 % barley or 55 % wheat
28.2.2005
Endo-1,3(4)-beta-glucanase
EC 3.2.1.6
Endo-1,3(4)-beta-glucanase:
11 000 BU
—

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Source: https://www.legislation.gov.uk/eur/2001/418/annex/II (the official version)
Page: https://openlegislation.uk/eu-regulation-2001-418/annex-II
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.