{
  "url": "https://openlegislation.uk/eu-regulation-2002-2188/annex-I",
  "json_url": "https://openlegislation.uk/eu-regulation-2002-2188/annex-I.json",
  "legislation": "eur/2002/2188",
  "legislation_title": "Commission Regulation (EC) No 2188/2002 of 9 December 2002 concerning the provisional authorisation of new uses of additives in feedingstuffs (Text with EEA relevance)",
  "legislation_url": "https://openlegislation.uk/eu-regulation-2002-2188",
  "path": "annex/I",
  "kind": "annex",
  "number": "I",
  "heading": null,
  "text": "1 U is the amount of enzyme which liberates 0,1 micromoles of glucose from carboxymethylcellulose per minute at pH 5,0 and 40 °C.\n1 U is the amount of enzyme which liberates 0,1 micromoles of glucose from barley beta-glucan per minute at pH 5,0 and 40 °C.\n1 U is the amount of enzyme which liberates 0,1 micromoles of glucose from oat spelt xylan per minute at pH 5,0 and 40 °C.\nNo (or EC No)\nAdditive\nChemical formula, description\nSpecies or category of animal\nMaximum age\nMinimum content\nMaximum content\nOther provisions\nEnd of period of authorization\nUnits of activity/kg of complete feedingstuff\nEnzymes\n11\nEndo-1,4-beta-glucanase\nEC 3.2.1.4\nPreparation of endo-1,4-beta-glucanase, endo-1,3(4)-beta-glucanase, and endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (ATCC 74252) having a minimum activity of:\nGranular and liquid form:\nEndo-1,4-beta-glucanase:\n8 000 U/g or ml\nEndo-1,3(4)-beta-glucanase:\n18 000 U/g or ml\nEndo-1,4-beta-xylanase:\n26 000 U/g or ml\nLaying hens\n—\nEndo-1,4-beta-glucanase:\n. . . . .\n—\n1.. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .\n2.. . . . . .\n3.. . . . . .\n1.1.2007\nEndo-1,3(4)-beta-glucanase\nEC 3.2.1.6\nEndo-1,3(4)-beta-gluca-nase:\n. . . . .\n—\nEndo-1,4-beta-xylanase\nEC 3.2.1.8\nEndo-1,4-beta-xylanase:\n. . . . .\n—\nPiglets\n—\nEndo-1,4-beta-gluca-nase:\n400 U\n—\n1.. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.\n2.. Recommended dosages per kilogram of complete feedingstuff:\nendo-1,4-beta-glucanase:\n400-1 600 U\nendo-1,3(4)-beta-glucanase:\n900-3 600 U\nendo-1, 4-beta-xylanase:\n1 300–5 200 U.\n3.. For use in compound feed rich in non-starch polysaccharides (mainly arabinoxylans and beta-glucans), e.g. containing more than 40 % wheat, triticale or maize or wheat and 20 % rye.\n1.1.2007\nEndo-1,3(4)-beta-glucanase:\n900 U\n—\nEndo-1,4-beta-xylanase:\n1 300 U\n—",
  "extent": "E+W+S+N.I.",
  "status": null,
  "version": "current",
  "annotations": [
    {
      "type": "F",
      "label": "F1",
      "text": "Deleted by Commission Implementing Regulation (EU) 2017/1145 of 8 June 2017 on the withdrawal from the market of certain feed additives authorised pursuant to Council Directives 70/524/EEC and 82/471/EEC and repealing the obsolete provisions authorising those feed additives (Text with EEA relevance)."
    },
    {
      "type": "footnote",
      "label": "f1",
      "text": "1 U is the amount of enzyme which liberates 0,1 micromoles of glucose from carboxymethylcellulose per minute at pH 5,0 and 40 °C."
    },
    {
      "type": "footnote",
      "label": "f2",
      "text": "1 U is the amount of enzyme which liberates 0,1 micromoles of glucose from barley beta-glucan per minute at pH 5,0 and 40 °C."
    },
    {
      "type": "footnote",
      "label": "f3",
      "text": "1 U is the amount of enzyme which liberates 0,1 micromoles of glucose from oat spelt xylan per minute at pH 5,0 and 40 °C."
    }
  ],
  "outstanding_effects": [],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/eur/2002/2188/annex/I",
    "retrieved_at": "2026-11-01T14:56:17.814641+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}