{
  "url": "https://openlegislation.uk/eu-regulation-2004-648/annex-IV/division-4/division-4.2/division-4.2.2",
  "json_url": "https://openlegislation.uk/eu-regulation-2004-648/annex-IV/division-4/division-4.2/division-4.2.2.json",
  "legislation": "eur/2004/648",
  "legislation_title": "Regulation (EC) No 648/2004 of the European Parliament and of the Council of 31 March 2004 on detergents (Text with EEA relevance)",
  "legislation_url": "https://openlegislation.uk/eu-regulation-2004-648",
  "path": "annex/IV/division/4/division/4.2/division/4.2.2",
  "kind": "division",
  "number": "4.2.2",
  "heading": "Effects on organisms. Tests to be conducted in compliance with the principles of good laboratory practice.",
  "text": "Fish: the test recommended is that in EU Regulation 440/2008, Annex, Part C, C.1. (acute toxicity for fish)\nDaphnia: the test recommended is that in EU Regulation 440/2008, Annex, Part C, C.2. (daphnia SP. acute immobilisation test)\nAlgae: the test recommended is that in EU Regulation 440/2008, Annex, Part C, C.3. (algal inhibition test)\nBacteria: the test recommended is that in EU Regulation 440/2008, Annex, Part C, C.11. (biodegradation – activated sludge respiration inhibition)",
  "extent": "E+W+S+N.I.",
  "status": null,
  "version": "current",
  "annotations": [
    {
      "type": "F",
      "label": "F1",
      "text": "Words in Annex 4 point 4.2.2 substituted (31.12.2020) by The Detergents (Amendment) (EU Exit) Regulations 2019 (S.I. 2019/672), regs. 1, 26(11)(a); 2020 c. 1, Sch. 5 para. 1(1)"
    },
    {
      "type": "F",
      "label": "F2",
      "text": "Words in Annex 4 point 4.2.2 substituted (31.12.2020) by The Detergents (Amendment) (EU Exit) Regulations 2019 (S.I. 2019/672), regs. 1, 26(11)(b); 2020 c. 1, Sch. 5 para. 1(1)"
    },
    {
      "type": "F",
      "label": "F3",
      "text": "Words in Annex 4 point 4.2.2 substituted (31.12.2020) by The Detergents (Amendment) (EU Exit) Regulations 2019 (S.I. 2019/672), regs. 1, 26(11)(c); 2020 c. 1, Sch. 5 para. 1(1)"
    },
    {
      "type": "F",
      "label": "F4",
      "text": "Words in Annex 4 point 4.2.2 substituted (31.12.2020) by The Detergents (Amendment) (EU Exit) Regulations 2019 (S.I. 2019/672), regs. 1, 26(11)(d); 2020 c. 1, Sch. 5 para. 1(1)"
    }
  ],
  "outstanding_effects": [],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/eur/2004/648/annex/IV/division/4/division/4.2/division/4.2.2",
    "retrieved_at": "2026-11-01T14:55:38.850310+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}