# Regulation (EC) No 648/2004 of the European Parliament and of the Council of 31 March 2004 on detergents (Text with EEA relevance), Division 4.2.2: Effects on organisms. Tests to be conducted in compliance with the principles of good laboratory practice.

Extent: E+W+S+N.I.

Fish: the test recommended is that in EU Regulation 440/2008, Annex, Part C, C.1. (acute toxicity for fish)
Daphnia: the test recommended is that in EU Regulation 440/2008, Annex, Part C, C.2. (daphnia SP. acute immobilisation test)
Algae: the test recommended is that in EU Regulation 440/2008, Annex, Part C, C.3. (algal inhibition test)
Bacteria: the test recommended is that in EU Regulation 440/2008, Annex, Part C, C.11. (biodegradation – activated sludge respiration inhibition)

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Source: https://www.legislation.gov.uk/eur/2004/648/annex/IV/division/4/division/4.2/division/4.2.2 (the official version)
Page: https://openlegislation.uk/eu-regulation-2004-648/annex-IV/division-4/division-4.2/division-4.2.2
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.