# Regulation (EC) No 648/2004 of the European Parliament and of the Council of 31 March 2004 on detergents (Text with EEA relevance), Division 4.4.1: Preparation of extract

Extent: E+W+S+N.I.

The quantity of surfactants necessary for the biodegradation test is about 50 g MBAS.
Normally, the quantity of product to be extracted will not exceed 1 000 g, but it may be necessary to extract further quantities of sample. For practical reasons, the quantity of product used should in most cases be limited to 5 000 g in preparing extracts for the biodegradation test.
Experience has shown that there are advantages in using a number of small extractions rather than one large extraction. The exchanger quantities specified are designed for a working capacity of 600-700 mmoles of surfactants and soap.

---
Source: https://www.legislation.gov.uk/eur/2004/648/annex/VIII/division/4/division/4.4/division/4.4.1 (the official version)
Page: https://openlegislation.uk/eu-regulation-2004-648/annex-VIII/division-4/division-4.4/division-4.4.1
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.