{
  "url": "https://openlegislation.uk/eu-regulation-2004-648/annex-VIII/division-4/division-4.4/division-4.4.2",
  "json_url": "https://openlegislation.uk/eu-regulation-2004-648/annex-VIII/division-4/division-4.4/division-4.4.2.json",
  "legislation": "eur/2004/648",
  "legislation_title": "Regulation (EC) No 648/2004 of the European Parliament and of the Council of 31 March 2004 on detergents (Text with EEA relevance)",
  "legislation_url": "https://openlegislation.uk/eu-regulation-2004-648",
  "path": "annex/VIII/division/4/division/4.4/division/4.4.2",
  "kind": "division",
  "number": "4.4.2",
  "heading": "Isolation of alcohol-soluble constituents",
  "text": "Add 250 g of the synthetic detergent to be analysed to 1 250 ml ethanol, heat the mixture to boiling point and reflux for one hour with stirring. Pass the hot alcoholic solution through a coarse-pored suction filter heated to 50 ° C and filter rapidly. Wash the flask and suction filter with approximately 200 ml hot ethanol. Collect the filtrate and filter washings in a filter flask.\nIn the case of pastes or liquid products to be analysed, make sure that not more than 55 g anionic surfactants and 35 g soap are contained in the sample. Evaporate this weighed sample to dryness. Dissolve the residue in 2 000 ml ethanol and proceed as described above. In the case of powders of low apparent density (< 300 g/l) it is recommended to increase the ethanol ratio in the relation 20:1. Evaporate the ethanolic filtrate to dryness, preferably by means of a rotary evaporator. Repeat the operation if a greater quantity of extract is required. Dissolve the residue in 5 000 ml isopropanol/water mixture.\nPreparation of ion-exchange columns",
  "extent": "E+W+S+N.I.",
  "status": null,
  "version": "current",
  "annotations": [],
  "outstanding_effects": [],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/eur/2004/648/annex/VIII/division/4/division/4.4/division/4.4.2",
    "retrieved_at": "2026-11-01T14:55:38.850310+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}