{
  "url": "https://openlegislation.uk/eu-regulation-2005-1812/annex-III",
  "json_url": "https://openlegislation.uk/eu-regulation-2005-1812/annex-III.json",
  "legislation": "eur/2005/1812",
  "legislation_title": "Commission Regulation (EC) No 1812/2005 of 4 November 2005 amending Regulations (EC) Nos 490/2004, 1288/2004, 521/2005 and 833/2005 as regards the conditions for the authorisation of certain additives in feedingstuffs belonging to the groups of enzymes and micro-organisms (Text with EEA relevance)",
  "legislation_url": "https://openlegislation.uk/eu-regulation-2005-1812",
  "path": "annex/III",
  "kind": "annex",
  "number": "III",
  "heading": null,
  "text": "1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 5,0 and 30 °C.\n1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from oat spelt xylan per minute at pH 5,3 and 50 °C.\n1 U is the amount of enzyme which liberates 1 microgram of phenolic compound (tyrosine equivalents) from a casein substrate per minute at pH 7,5 and 40 °C.\nEC No\nAdditive\nChemical formula, description\nSpecies or category of animal\nMaximum age\nMinimum content\nMaximum content\nOther provisions\nEnd of period of authorisation\nUnits of activity/kg of complete feedingstuff\nEnzymes\nE 1623\nEndo-1,3(4)-beta-glucanase EC 3.2.1.6\nEndo-1,4-beta-xylanase EC 3.2.1.8\nSubtilisin EC 3.4.21.62\nPreparation of endo-1,3(4)-beta-glucanase produced by Trichoderma longibrachiatum (ATCC 2106), endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (ATCC 2105) and subtilisin produced by Bacillus subtilis (ATCC 2107) having a minimum activity of:\nendo-1,3(4)-beta-glucanase: 200 U/g\nendo-1,4-beta-xylanase: 5 000 U/g\nsubtilisin: 1 600 U/g\nChickens for fattening\n—\nendo-1,3(4)-beta-glucanase: 25 U\n—\n1.\nIn the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.\n2.\nRecommended dose per kg of complete feedingstuff:\nendo-1,3(4)-beta-glucanase: 25-100 U\nendo-1,4-beta-xylanase: 625-2 500 U\nsubtilisin: 200-800 U.\n3.\nFor use in compound feed, e.g. containing more than 30 % wheat and 10 % barley.\nWithout a time-limit\nendo-1,4-beta-xylanase: 625 U\n—\nsubtilisin: 200 U\n—",
  "extent": "E+W+S+N.I.",
  "status": null,
  "version": "current",
  "annotations": [
    {
      "type": "footnote",
      "label": "f1",
      "text": "1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 5,0 and 30 °C."
    },
    {
      "type": "footnote",
      "label": "f2",
      "text": "1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from oat spelt xylan per minute at pH 5,3 and 50 °C."
    },
    {
      "type": "footnote",
      "label": "f3",
      "text": "1 U is the amount of enzyme which liberates 1 microgram of phenolic compound (tyrosine equivalents) from a casein substrate per minute at pH 7,5 and 40 °C."
    }
  ],
  "outstanding_effects": [],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/eur/2005/1812/annex/III",
    "retrieved_at": "2026-11-01T14:54:10.443553+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}