# Commission Regulation (EC) No 1812/2005 of 4 November 2005 amending Regulations (EC) Nos 490/2004, 1288/2004, 521/2005 and 833/2005 as regards the conditions for the authorisation of certain additives in feedingstuffs belonging to the groups of enzymes and micro-organisms (Text with EEA relevance), Annex III

Extent: E+W+S+N.I.

1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 5,0 and 30 °C.
1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from oat spelt xylan per minute at pH 5,3 and 50 °C.
1 U is the amount of enzyme which liberates 1 microgram of phenolic compound (tyrosine equivalents) from a casein substrate per minute at pH 7,5 and 40 °C.
EC No
Additive
Chemical formula, description
Species or category of animal
Maximum age
Minimum content
Maximum content
Other provisions
End of period of authorisation
Units of activity/kg of complete feedingstuff
Enzymes
E 1623
Endo-1,3(4)-beta-glucanase EC 3.2.1.6
Endo-1,4-beta-xylanase EC 3.2.1.8
Subtilisin EC 3.4.21.62
Preparation of endo-1,3(4)-beta-glucanase produced by Trichoderma longibrachiatum (ATCC 2106), endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (ATCC 2105) and subtilisin produced by Bacillus subtilis (ATCC 2107) having a minimum activity of:
endo-1,3(4)-beta-glucanase: 200 U/g
endo-1,4-beta-xylanase: 5 000 U/g
subtilisin: 1 600 U/g
Chickens for fattening
—
endo-1,3(4)-beta-glucanase: 25 U
—
1.
In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.
2.
Recommended dose per kg of complete feedingstuff:
endo-1,3(4)-beta-glucanase: 25-100 U
endo-1,4-beta-xylanase: 625-2 500 U
subtilisin: 200-800 U.
3.
For use in compound feed, e.g. containing more than 30 % wheat and 10 % barley.
Without a time-limit
endo-1,4-beta-xylanase: 625 U
—
subtilisin: 200 U
—

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Source: https://www.legislation.gov.uk/eur/2005/1812/annex/III (the official version)
Page: https://openlegislation.uk/eu-regulation-2005-1812/annex-III
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.