# Commission Regulation (EC) No 1812/2005 of 4 November 2005 amending Regulations (EC) Nos 490/2004, 1288/2004, 521/2005 and 833/2005 as regards the conditions for the authorisation of certain additives in feedingstuffs belonging to the groups of enzymes and micro-organisms (Text with EEA relevance), Annex IV

Extent: E+W+S+N.I.

In the Annex to Regulation (EC) No 833/2005, the entry for E 1627 is replaced by the following:
1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 5,0 and 30 °C.
1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from oat spelt xylan per minute at pH 5,3 and 50 °C.’
‘E 1627
Endo-1,3(4)-beta-glucanase EC 3.2.1.6
Endo-1,4-beta-xylanase EC 3.2.1.8
Preparation of endo-1,3(4)-beta-glucanase produced by Trichoderma longibrachiatum (ATCC 2106) and endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (ATCC 2105) having a minimum activity of:
powder form:
endo-1,3(4)-beta-glucanase: 800 U/g
endo-1,4-beta-xylanase: 800 U/g
liquid form:
endo-1,3(4)-beta-glucanase: 800 U/ml
endo-1,4-beta-xylanase: 800 U/ml
Pigs for fattening
—
endo-1,3(4)-beta-glucanase: 400 U
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1.
In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.
2.
Recommended dose per kg of complete feedingstuff:
endo-1,3(4)-beta-glucanase: 400 U
endo-1,4-beta-xylanase: 400 U.
3.
For use in compound feed rich in non-starch polysaccharides (mainly beta-glucans and arabinoxylans) e.g. containing more than 65 % barley.
Without a time-limit
endo-1,4-beta-xylanase: 400 U
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Source: https://www.legislation.gov.uk/eur/2005/1812/annex/IV (the official version)
Page: https://openlegislation.uk/eu-regulation-2005-1812/annex-IV
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.