{
  "url": "https://openlegislation.uk/eu-regulation-2005-2036/annex-III",
  "json_url": "https://openlegislation.uk/eu-regulation-2005-2036/annex-III.json",
  "legislation": "eur/2005/2036",
  "legislation_title": "Commission Regulation (EC) No 2036/2005 of 14 December 2005 concerning the permanent authorisations of certain additives in feedingstuffs and the provisional authorisation of a new use of certain additives already authorised in feedingstuffs (Text with EEA relevance)",
  "legislation_url": "https://openlegislation.uk/eu-regulation-2005-2036",
  "path": "annex/III",
  "kind": "annex",
  "number": "III",
  "heading": null,
  "text": "1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from oat spelt xylan per minute at pH 5,3 and 50 °C.\n1 U is the amount of enzyme which liberates 1 microgram of phenolic compound (tyrosine equivalents) from a casein substrate per minute at pH 7,5 and 40 °C.\n1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 5,0 and 30 °C.\n1 U is the amount of enzyme which hydrolyses 1 micromole of glucosidic linkages from a water insoluble cross-linked starch polymer substrate per minute at pH 6,5 and 37 °C.\n1 U is the amount of enzyme which liberates 1 micromole of reducing material (galacturonic acid equivalents) from a poly D-galacturonic substrate per minute at pH 5,0 and 40 °C.\nEC No or No\nAdditive\nChemical formula, description\nSpecies or category of animal\nMaximum age\nMinimum content\nMaximum content\nOther provisions\nEnd of period of authorisation\nUnits of activity/kg of complete feedingstuff\nEnzymes\n37\nEndo-1,4-beta-xylanase\nEC 3.2.1.8\nSubtilisin\nEC 3.4.21.62\nPreparation of endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (ATCC 2105) and subtilisin produced by Bacillus subtilis (ATCC 2107) having a minimum activity of:\nEndo-1,4-beta-xylanase: 5 000 U/g\nSubtilisin: 1 600 U/g\nDucks\n—\nendo-1,4-beta-xylanase:\n2 500 U\n—\n1.\nIn the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.\n2.\nRecommended dose per kg of complete feedingstuff:\nendo-1,4-beta-xylanase: 2 500 U\nsubtilisin: 800 U.\n3.\nFor use in compound feed e.g. containing more than 65 % wheat.\n4.1.2010\nsubtilisin:\n800 U\n—\n59\nEndo-1,4-beta-xylanase\nEC 3.2.1.8\nEndo-1,3(4)-beta-glucanase\nEC 3.2.1.6\nSubtilisin\nEC 3.4.21.62\nAlpha-amylase\nEC 3.2.1.1\nPolygalacturonase\nEC 3.2.1.15\nPreparation of endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (ATCC 2105), endo-1,3(4)-beta-glucanase and alpha-amylase produced by Bacillus amyloliquefaciens (DSM 9553), subtilisin produced by Bacillus subtilis (ATCC 2107), polygalacturonase produced by Aspergillus aculeatus (CBS 589.94) having a mimimum activity of:\nEndo-1,4-beta-xylanase: 300 U/g\nEndo-1,3(4)-beta-glucanase: 150 U/g\nSubtilisin: 4 000 U/g\nAlpha-amylase: 400 U/g\nPolygalacturonase: 25 U/g\nDucks\n—\nendo-1,4-beta-xylanase:\n300 U\n—\n1.\nIn the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.\n2.\nRecommended dose per kg of complete feedingstuff:\nendo-1,4-beta-xylanase: 300 U\nendo-1,3(4)-beta-glucanase: 150 U\nsubtilisin: 4 000 U\nalpha-amylase: 400 U\npolygalacturonase: 25 U.\n3.\nFor use in compound feed rich in starch and non-starch polysaccharides (mainly arabinoxylans and beta-glucans), e.g. containing more than 40 % maize.\n4.1.2010\nendo-1,3(4)-beta-glucanase:\n150 U\n—\nsubtilisin:\n4 000 U\n—\nalpha-amylase:\n400 U\n—\npolygalacturonase:\n25 U\n—\nLaying hens\n—\nendo-1,4-beta-xylanase:\n225 U\n—\n1.\nIn the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.\n2.\nRecommended dose per kg of complete feedingstuff:\nendo-1,4-beta-xylanase: 225 U\nendo-1,3(4)-beta-glucanase: 112 U\nsubtilisin: 3 000 U\nalpha-amylase: 300 U\npolygalacturonase: 18 U.\n3.\nFor use in compound feed rich in starch and non-starch polysaccharides (mainly arabinoxylans and beta-glucans), e.g. containing more than 40 % maize.\n4.1.2010\nendo-1,3(4)-beta-glucanase:\n112 U\n—\nsubtilisin:\n3 000 U\n—\nalpha-amylase:\n300 U\n—\npolygalac-turonase:\n18 U\n—",
  "extent": "E+W+S+N.I.",
  "status": null,
  "version": "current",
  "annotations": [
    {
      "type": "F",
      "label": "F1",
      "text": "Deleted by Commission Implementing Regulation (EU) No 1206/2012 of 14 December 2012 concerning the authorisation of a preparation of endo-1,4-beta-xylanase produced by Aspergillus oryzae (DSM 10287) as a feed additive for poultry for fattening, weaned piglets and pigs for fattening and amending Regulations (EC) No 1332/2004 and (EC) No 2036/2005 (holder of the authorisation DSM Nutritional Products) (Text with EEA relevance)."
    },
    {
      "type": "footnote",
      "label": "f1",
      "text": "1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from oat spelt xylan per minute at pH 5,3 and 50 °C."
    },
    {
      "type": "footnote",
      "label": "f2",
      "text": "1 U is the amount of enzyme which liberates 1 microgram of phenolic compound (tyrosine equivalents) from a casein substrate per minute at pH 7,5 and 40 °C."
    },
    {
      "type": "footnote",
      "label": "f3",
      "text": "1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 5,0 and 30 °C."
    },
    {
      "type": "footnote",
      "label": "f4",
      "text": "1 U is the amount of enzyme which hydrolyses 1 micromole of glucosidic linkages from a water insoluble cross-linked starch polymer substrate per minute at pH 6,5 and 37 °C."
    },
    {
      "type": "footnote",
      "label": "f5",
      "text": "1 U is the amount of enzyme which liberates 1 micromole of reducing material (galacturonic acid equivalents) from a poly D-galacturonic substrate per minute at pH 5,0 and 40 °C."
    }
  ],
  "outstanding_effects": [],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/eur/2005/2036/annex/III",
    "retrieved_at": "2026-11-01T14:54:05.075221+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}