# Commission Regulation (EC) No 2036/2005 of 14 December 2005 concerning the permanent authorisations of certain additives in feedingstuffs and the provisional authorisation of a new use of certain additives already authorised in feedingstuffs (Text with EEA relevance), Annex III

Extent: E+W+S+N.I.

1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (xylose equivalents) from oat spelt xylan per minute at pH 5,3 and 50 °C.
1 U is the amount of enzyme which liberates 1 microgram of phenolic compound (tyrosine equivalents) from a casein substrate per minute at pH 7,5 and 40 °C.
1 U is the amount of enzyme which liberates 1 micromole of reducing sugars (glucose equivalents) from barley beta-glucan per minute at pH 5,0 and 30 °C.
1 U is the amount of enzyme which hydrolyses 1 micromole of glucosidic linkages from a water insoluble cross-linked starch polymer substrate per minute at pH 6,5 and 37 °C.
1 U is the amount of enzyme which liberates 1 micromole of reducing material (galacturonic acid equivalents) from a poly D-galacturonic substrate per minute at pH 5,0 and 40 °C.
EC No or No
Additive
Chemical formula, description
Species or category of animal
Maximum age
Minimum content
Maximum content
Other provisions
End of period of authorisation
Units of activity/kg of complete feedingstuff
Enzymes
37
Endo-1,4-beta-xylanase
EC 3.2.1.8
Subtilisin
EC 3.4.21.62
Preparation of endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (ATCC 2105) and subtilisin produced by Bacillus subtilis (ATCC 2107) having a minimum activity of:
Endo-1,4-beta-xylanase: 5 000 U/g
Subtilisin: 1 600 U/g
Ducks
—
endo-1,4-beta-xylanase:
2 500 U
—
1.
In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.
2.
Recommended dose per kg of complete feedingstuff:
endo-1,4-beta-xylanase: 2 500 U
subtilisin: 800 U.
3.
For use in compound feed e.g. containing more than 65 % wheat.
4.1.2010
subtilisin:
800 U
—
59
Endo-1,4-beta-xylanase
EC 3.2.1.8
Endo-1,3(4)-beta-glucanase
EC 3.2.1.6
Subtilisin
EC 3.4.21.62
Alpha-amylase
EC 3.2.1.1
Polygalacturonase
EC 3.2.1.15
Preparation of endo-1,4-beta-xylanase produced by Trichoderma longibrachiatum (ATCC 2105), endo-1,3(4)-beta-glucanase and alpha-amylase produced by Bacillus amyloliquefaciens (DSM 9553), subtilisin produced by Bacillus subtilis (ATCC 2107), polygalacturonase produced by Aspergillus aculeatus (CBS 589.94) having a mimimum activity of:
Endo-1,4-beta-xylanase: 300 U/g
Endo-1,3(4)-beta-glucanase: 150 U/g
Subtilisin: 4 000 U/g
Alpha-amylase: 400 U/g
Polygalacturonase: 25 U/g
Ducks
—
endo-1,4-beta-xylanase:
300 U
—
1.
In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.
2.
Recommended dose per kg of complete feedingstuff:
endo-1,4-beta-xylanase: 300 U
endo-1,3(4)-beta-glucanase: 150 U
subtilisin: 4 000 U
alpha-amylase: 400 U
polygalacturonase: 25 U.
3.
For use in compound feed rich in starch and non-starch polysaccharides (mainly arabinoxylans and beta-glucans), e.g. containing more than 40 % maize.
4.1.2010
endo-1,3(4)-beta-glucanase:
150 U
—
subtilisin:
4 000 U
—
alpha-amylase:
400 U
—
polygalacturonase:
25 U
—
Laying hens
—
endo-1,4-beta-xylanase:
225 U
—
1.
In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.
2.
Recommended dose per kg of complete feedingstuff:
endo-1,4-beta-xylanase: 225 U
endo-1,3(4)-beta-glucanase: 112 U
subtilisin: 3 000 U
alpha-amylase: 300 U
polygalacturonase: 18 U.
3.
For use in compound feed rich in starch and non-starch polysaccharides (mainly arabinoxylans and beta-glucans), e.g. containing more than 40 % maize.
4.1.2010
endo-1,3(4)-beta-glucanase:
112 U
—
subtilisin:
3 000 U
—
alpha-amylase:
300 U
—
polygalac-turonase:
18 U
—

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Source: https://www.legislation.gov.uk/eur/2005/2036/annex/III (the official version)
Page: https://openlegislation.uk/eu-regulation-2005-2036/annex-III
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.