{
  "url": "https://openlegislation.uk/eu-regulation-2006-1664/annex-III",
  "json_url": "https://openlegislation.uk/eu-regulation-2006-1664/annex-III.json",
  "legislation": "eur/2006/1664",
  "legislation_title": "Commission Regulation (EC) No 1664/2006 of 6 November 2006 amending Regulation (EC) No 2074/2005 as regards implementing measures for certain products of animal origin intended for human consumption and repealing certain implementing measures (Text with EEA relevance)",
  "legislation_url": "https://openlegislation.uk/eu-regulation-2006-1664",
  "path": "annex/III",
  "kind": "annex",
  "number": "III",
  "heading": null,
  "text": "The following Annex VIa on testing methods for raw milk and heat-treated milk is added to Regulation (EC) No 2074/2005:\nANNEX VIa\nTESTING METHODS FOR RAW MILK AND HEAT-TREATED MILK\nCHAPTER I\nDETERMINATION OF PLATE COUNT AND SOMATIC CELL COUNT\n1.\nWhen checking against the criteria laid down in Annex III, Section IX, Chapter I, Part III to Regulation (EC) No 853/2004, the following standards must be applied as reference methods:\n(a) EN/ISO 4833 for the plate count at 30 oC;\n(b) ISO 13366-1 for the somatic cell count.\n2.\nThe use of alternative analytical methods is acceptable:\n(a) For the plate count at 30 oC, when the methods are validated against the reference method mentioned in point 1(a) in accordance with the protocol set out in EN/ISO standard 16140 or other similar internationally accepted protocols.\nIn particular the conversion relationship between an alternative method and the reference method mentioned in point 1(a) is established according to ISO standard 21187.\n(b) For the somatic cell count, when the methods are validated against the reference method mentioned in point 1(b) in accordance with the protocol set out in ISO 8196 and when operated in accordance with ISO standard 13366-2 or other similar internationally accepted protocols.\nCHAPTER II\nDETERMINATION OF ALKALINE PHOSPHATASE ACTIVITY\n1.\nWhen determining alkaline phosphatase activity, ISO standard 11816-1 must be applied as reference method.\n2.\nThe alkaline phosphatase activity is expressed as milliunits of enzyme activity per litre (mU/l). A unit of alkaline phosphatase activity is the amount of alkaline phosphatase enzyme that catalyses the transformation of 1 micromole of substrate per minute.\n3.\nAn alkaline phosphatase test is considered to give a negative result if the measured activity in cow's milk is not higher than 350 mU/l.\n4.\nThe use of alternative analytical methods is acceptable when the methods are validated against the reference method mentioned in point 1 in accordance with internationally accepted protocols.",
  "extent": "E+W+S+N.I.",
  "status": null,
  "version": "current",
  "annotations": [],
  "outstanding_effects": [],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/eur/2006/1664/annex/III",
    "retrieved_at": "2026-11-01T14:53:21.873860+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}