{
  "url": "https://openlegislation.uk/eu-regulation-2008-164/annex",
  "json_url": "https://openlegislation.uk/eu-regulation-2008-164/annex.json",
  "legislation": "eur/2008/164",
  "legislation_title": "Commission Regulation (EC) No 164/2008 of 22 February 2008 amending Regulation (EC) No 1444/2006 as regards the minimum content of the feed additive Bacillus subtilis C-3102 (Calsporin) (Text with EEA relevance)",
  "legislation_url": "https://openlegislation.uk/eu-regulation-2008-164",
  "path": "annex",
  "kind": "annex",
  "number": null,
  "heading": null,
  "text": "‘ANNEX\nDetails of the analytical methods are available at the following address of the Community Reference Laboratory: www.irmm.jrc.be/crl-feed-additives’\nIdentification number of the additive\nName of the holder of authorisation\nAdditive(Trade name)\nComposition, chemical formula, description, analytical method\nSpecies or category of animal\nMaximum age\nMinimum content\nMaximum content\nOther provisions\nEnd of period of authorisation\nCFU/kg of complete feedingstuff with a moisture content of 12 %\nCategory of zootechnical additives. Functional group: gut flora stabilisers\n4b1820\nCalpis Co., Ltd\nRepresented in the Community by Orffa International Holding BV\nBacillus subtilis\nC-3102 DSM 15544\n(Calsporin)\nAdditive composition:\nPreparation of Bacillus subtilis C-3102 (DSM 15544) containing a minimum of 1 × 1010 CFU/g of additive\nCharacterisation of the active substance:\nViable spores of Bacillus subtilis C-3102 (DSM 15544)\nAnalytical method\nEnumeration spread plate method using tryptone soya agar with preheat treatment of feed samples\nChickens for fattening\n—\n5 × 108\n1 × 109\n1.\nFor user safety: breathing protection during handling and safety glasses.\n2.\nIn the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.\n3.\nThe use is permitted in feed containing the permitted coccidiostats: monensin sodium, salinomycin sodium, semduramycin sodium, lasalocid sodium, maduramycin ammonium, narasin-nicarbazin and diclazuril.\n20 October 2016",
  "extent": "E+W+S+N.I.",
  "status": null,
  "version": "current",
  "annotations": [
    {
      "type": "footnote",
      "label": "f1",
      "text": "Details of the analytical methods are available at the following address of the Community Reference Laboratory: www.irmm.jrc.be/crl-feed-additives’"
    }
  ],
  "outstanding_effects": [],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/eur/2008/164/annex",
    "retrieved_at": "2026-11-01T14:52:25.016539+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}