# Commission Regulation (EC) No 900/2008 of 16 September 2008 laying down the methods of analysis and other technical provisions necessary for the application of the arrangements for imports of certain goods resulting from the processing of agricultural products (Codified version), Division 3: Principle

Extent: E+W+S+N.I.

The samples are homogenised and suspended in water. The starch and its degradation products, present in the samples, are enzymatically converted into glucose in two steps:
1.. Starch and its degradation products are partially converted into soluble glucose chains using thermostable alpha-amylase at 90 °C. For effective conversion it is necessary that the samples should be completely solved or should be present in the form of a suspension containing very small solid parts
2.. The soluble glucose chains are converted into glucose using amyloglucosidase at 60 °C.
Products containing a high content of proteins or fat are clarified and filtrated.
The determination of sugars is performed by HPLC analysis.
Because a partial inversion of sucrose may occur during the enzymatic treatment, the determination of free sugars is also performed by HPLC analysis to calculate the corrected glucose content.

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Source: https://www.legislation.gov.uk/eur/2008/900/annex/I/division/3 (the official version)
Page: https://openlegislation.uk/eu-regulation-2008-900/annex-I/division-3
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.